FLNB (Filamin-B)/FLN1L/FLN3/TABP/TAP/FLN-B/Fh1/Truncated ABP/Filamin-3/Filamin homolog 1/Beta-filamin/Actin-binding-like protein/Truncated actin-binding protein/Thyroid autoantigen/ABP-278/ABP-280 homolog

For Research Use Only. Not for use in diagnostic procedures.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of FLNB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS762944 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Filamin-B (FLNB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FLNB. The ELISA analytical biochemical technique of the MBS762944 kit is based on FLNB antibody-FLNB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FLNB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FLNB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-FLNB antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-FLNB antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the FLNB amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of FLNB can be calculated.

276,939 Da

filamin B

filamin-B

Filamin-B

FLN1L; FLN3; TABP; TAP; FLN-B; Fh1; Truncated ABP??[Similar Products]

FLNB_HUMAN

This gene encodes a member of the filamin family. The encoded protein interacts with glycoprotein Ib alpha as part of the process to repair vascular injuries. The platelet glycoprotein Ib complex includes glycoprotein Ib alpha, and it binds the actin cytoskeleton. Mutations in this gene have been found in several conditions: atelosteogenesis type 1 and type 3; boomerang dysplasia; autosomal dominant Larsen syndrome; and spondylocarpotarsal synostosis syndrome. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Nov 2009]

FLNB: a ubiquitous cytoskeletal protein that links actin filaments to membrane glycoproteins. Six alternatively spliced isoforms have been described. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. Isoforms 1 and 2 are expressed in placenta, bone marrow, brain, umbilical vein endothelial cells, retina and skeletal muscle. Isoform 1 is predominantly expressed in prostate, uterus, liver, thyroid, stomach, lymph node, small intestine, spleen, skeletal muscle, kidney, placenta, pancreas, heart, lung, platelets, endothelial cells, megakaryocytic and erythroleukemic cell lines. Isoform 2 is predominantly expressed in spinal cord and platelets. Also expressed in thyroid adenoma, neurofibrillary tangles (NFT), senile plaques in the hippocampus and cerebral cortex in Alzheimer's disease. Isoform 3 and isoform 6 are expressed predominantly in lung, heart, skeletal muscle, testis, spleen, thymus and leukocytes. Isoform 4 and isoform 5 are expressed in heart.

Protein type: Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3p14.3

Cellular Component: actin cytoskeleton; brush border; cell cortex; cytoplasm; cytosol; focal adhesion; integral to membrane; plasma membrane; stress fiber; Z disc

Molecular Function: actin binding; identical protein binding; protein binding

Biological Process: actin cytoskeleton organization and biogenesis; cytokine and chemokine mediated signaling pathway; cytoskeletal anchoring; signal transduction; skeletal muscle development

Disease: Atelosteogenesis, Type I; Atelosteogenesis, Type Iii; Boomerang Dysplasia; Larsen Syndrome; Spondylocarpotarsal Synostosis Syndrome

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