BDA1; HHG2

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Hedgehog Homolog, Indian (IHH).
No significant cross-reactivity or interference between Hedgehog Homolog, Indian (IHH) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of IHH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2704925 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Hedgehog Homolog, Indian (IHH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IHH. The ELISA analytical biochemical technique of the MBS2704925 kit is based on IHH antibody-IHH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IHH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IHH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hedgehog Homolog, Indian (IHH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hedgehog Homolog, Indian (IHH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hedgehog Homolog, Indian (IHH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hedgehog Homolog, Indian (IHH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

45,251 Da

indian hedgehog

indian hedgehog protein

Indian hedgehog protein

IHH??[Similar Products]

This gene encodes a member of the hedgehog family of proteins. The encoded preproprotein is proteolytically processed to generate multiple protein products, including an N-terminal fragment that is involved in signaling. Hedgehog family proteins are essential secreted signaling molecules that regulate a variety of developmental processes including growth, patterning and morphogenesis. The protein encoded by this gene specifically plays a role in bone growth and differentiation. Mutations in this gene are the cause of brachydactyly type A1, which is characterized by shortening or malformation of the fingers and toes. Mutations in this gene are also the cause of acrocapitofemoral dysplasia. [provided by RefSeq, Nov 2015]

IHH: Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP). Expressed in embryonic lung, and in ***** kidney and liver. Belongs to the hedgehog family.

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: extracellular space; plasma membrane; proteinaceous extracellular matrix

Molecular Function: calcium ion binding; patched binding; peptidase activity

Biological Process: bone resorption; branching involved in blood vessel morphogenesis; cell fate specification; cell maturation; cell-cell signaling; embryonic camera-type eye morphogenesis; embryonic digestive tract morphogenesis; embryonic digit morphogenesis; embryonic pattern specification; heart looping; in utero embryonic development; intein-mediated protein splicing; maternal process involved in female pregnancy; multicellular organism growth; negative regulation of alpha-beta T cell differentiation; negative regulation of apoptosis; negative regulation of eye pigmentation; negative regulation of immature T cell proliferation in the thymus; negative regulation of T cell differentiation in thymus; neuron development; osteoblast differentiation; pancreas development; positive regulation of alpha-beta T cell differentiation; positive regulation of collagen biosynthetic process; positive regulation of epithelial cell proliferation; positive regulation of mesenchymal cell proliferation; positive regulation of smoothened signaling pathway; positive regulation of T cell differentiation in thymus; positive regulation of transcription from RNA polymerase II promoter; proteoglycan metabolic process; regulation of growth; response to estradiol; response to mechanical stimulus; skeletal system development; smooth muscle development; smoothened signaling pathway; vitelline membrane formation

Disease: Acrocapitofemoral Dysplasia; Brachydactyly, Type A1

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