Product Name
CD295 (LEPR), Polyclonal Antibody
Full Product Name
Anti-CD295 Antibody
Product Synonym Names
DB; OBR; Leptin receptor; LEP-R; HuB219; OB receptor; OB-R; CD295
Product Gene Name
anti-LEPR antibody
[Similar Products]
Antibody/Peptide Pairs
CD295 peptide (MBS823462) is used for blocking the activity of CD295 antibody (MBS821996)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P48357
Specificity
Recognizes endogenous levels of CD295 protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD295. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-LEPR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-LEPR antibody
Rabbit polyclonal antibody to CD295
Applications Tested/Suitable for anti-LEPR antibody
Western Blot (WB)
Application Notes for anti-LEPR antibody
WB (1/500 - 1/1000)
Western Blot (WB) of anti-LEPR antibody
Western blot analysis of CD295 expression in MCF7 (A), HepG2 (B), HeLa (C) whole cell lysates.
Immunohistochemistry (IHC) of anti-LEPR antibody
NCBI/Uniprot data below describe general gene information for LEPR. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001003679.1
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NCBI GenBank Nucleotide #
NM_001003679.3
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UniProt Primary Accession #
P48357
[Other Products]
UniProt Secondary Accession #
Q13592; Q13593; Q13594; Q92919; Q92920; Q92921[Other Products]
UniProt Related Accession #
P48357[Other Products]
Molecular Weight
96,042 Da
NCBI Official Full Name
leptin receptor isoform 3
NCBI Official Synonym Full Names
leptin receptor
NCBI Official Symbol
LEPR??[Similar Products]
NCBI Official Synonym Symbols
OBR; OB-R; CD295; LEP-R; LEPRD
??[Similar Products]
NCBI Protein Information
leptin receptor; huB219; OB receptor
UniProt Protein Name
Leptin receptor
UniProt Synonym Protein Names
HuB219; OB receptor; OB-R; CD_antigen: CD295
Protein Family
Leptin receptor
UniProt Gene Name
LEPR??[Similar Products]
UniProt Synonym Gene Names
DB; OBR; LEP-R; OB-R??[Similar Products]
UniProt Entry Name
LEPR_HUMAN
NCBI Summary for LEPR
The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).[provided by RefSeq, Nov 2010]
UniProt Comments for LEPR
LEPR: the receptor for leptin (obesity factor). A single-transmembrane-domain receptor of the cytokine receptor family. On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Five alternatively spliced transcript variants have been reported.
Protein type: Membrane protein, integral; Receptor, cytokine
Chromosomal Location of Human Ortholog: 1p31
Cellular Component: plasma membrane; integral to membrane; extracellular region; receptor complex
Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; identical protein binding; protein binding; transmembrane receptor activity
Biological Process: cholesterol metabolic process; cell surface receptor linked signal transduction; multicellular organismal development; cytokine and chemokine mediated signaling pathway; negative regulation of hydrolase activity; negative regulation of gluconeogenesis; energy reserve metabolic process
Disease: Leptin Receptor Deficiency
Research Articles on LEPR
1. findings suggested lack of association between LEPR Q223R polymorphisms and cancer susceptibility, but the LEPR Q223R genetic polymorphism may increase the susceptibility to cancers in Asian and African individuals
Precautions
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Disclaimer
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