Product Name
Hedgehog Homolog, Indian (IHH), DIY ELISA Kit
Full Product Name
Hedgehog Homolog, Indian (IHH) ELISA Kit DIY Materials
Product Synonym Names
BDA1; HHG2
Product Gene Name
IHH diy elisa kit
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Request Current Manual
OMIM
AB018076 Genomic DNA
3D Structure
ModBase 3D Structure for Q14623
Specificity
The Abs in the kit have high sensitivity and excellent specificity for detection of Hedgehog Homolog, Indian (IHH). No significant cross-reactivity or interference between Hedgehog Homolog, Indian (IHH) and analogues was observed.
Assay Type
Double-antibody Sandwich ELISA for Antigen Detection
Samples
Tissue homogenates, cell lysates and other
biological fluids.
Detection Range
0.156-10ng/mL
Reagent Contents
Capture
Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Product Usage
1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Other Reagents Required
ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IHH diy elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for IHH diy elisa kit
Signal transduction
Application Notes for IHH diy elisa kit
Main materials for "Do It Yourself ELISA Kit"
NCBI/Uniprot data below describe general gene information for IHH. It may not necessarily be applicable to this product.
NCBI Accession #
NP_002172.2
[Other Products]
NCBI GenBank Nucleotide #
NM_002181.3
[Other Products]
UniProt Primary Accession #
Q14623
[Other Products]
UniProt Secondary Accession #
O43322; Q8N4B9; B9EGM5[Other Products]
UniProt Related Accession #
Q14623[Other Products]
Molecular Weight
45,251 Da
NCBI Official Full Name
indian hedgehog protein preproprotein
NCBI Official Synonym Full Names
indian hedgehog
NCBI Official Symbol
IHH??[Similar Products]
NCBI Official Synonym Symbols
BDA1; HHG2
??[Similar Products]
NCBI Protein Information
indian hedgehog protein
UniProt Protein Name
Indian hedgehog protein
UniProt Synonym Protein Names
HHG-2
Protein Family
Indian hedgehog protein
UniProt Gene Name
IHH??[Similar Products]
UniProt Synonym Gene Names
IHH??[Similar Products]
NCBI Summary for IHH
This gene encodes a member of the hedgehog family of proteins. The encoded preproprotein is proteolytically processed to generate multiple protein products, including an N-terminal fragment that is involved in signaling. Hedgehog family proteins are essential secreted signaling molecules that regulate a variety of developmental processes including growth, patterning and morphogenesis. The protein encoded by this gene specifically plays a role in bone growth and differentiation. Mutations in this gene are the cause of brachydactyly type A1, which is characterized by shortening or malformation of the fingers and toes. Mutations in this gene are also the cause of acrocapitofemoral dysplasia. [provided by RefSeq, Nov 2015]
UniProt Comments for IHH
IHH: Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP). Expressed in embryonic lung, and in ***** kidney and liver. Belongs to the hedgehog family.
Chromosomal Location of Human Ortholog: 2q35
Cellular Component: plasma membrane
Molecular Function: calcium ion binding; patched binding
Biological Process: heart looping; negative regulation of alpha-beta T cell differentiation; negative regulation of immature T cell proliferation in the thymus; negative regulation of T cell differentiation in the thymus; positive regulation of alpha-beta T cell differentiation; positive regulation of smoothened signaling pathway; positive regulation of T cell differentiation in the thymus; skeletal development; smoothened signaling pathway
Disease: Acrocapitofemoral Dysplasia; Brachydactyly, Type A1
Research Articles on IHH
1. The serum levels of IHH and SHH were significantly higher in autistic subjects than those of control subjects. The findings support a correlation between SHH, IHH and BDNF in autistic children, suggesting their pathological role in autism.
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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