Acyl-coenzyme A thioesterase 8; hACTE-III; HNAACTE; the; PTE-1; PTE-2; PTE1; PTE2

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG2a, k

AT4D10

Mouse

By protein-A affinity chromatography

Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.

1 mg/ml (lot specific)

Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.

Small volumes of anti-ACOT8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Acyl-coenzyme A thioesterase 8 is an enzyme that in humans is encoded by the ACOT8 gene. The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. Multiple transcript variants encoding several different isoforms have been found for this gene.

Signal TransductionM; Metabolism; Cancer; Immunology

ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

The Recombinant Human ACOT8 (10ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACOT8 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human ACOT8 Lane 2.: Ramos cell lysate Lane 3.: A431 cell lysate Lane 4 : 293T cell lysate Lane 5 : A549 cell lysate

ICC/IF analysis of ACOT8 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACOT8 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

35,914 Da

acyl-CoA thioesterase 8

acyl-coenzyme A thioesterase 8

Acyl-coenzyme A thioesterase 8

ACTEIII; PTE1; PTE2; Acyl-CoA thioesterase 8; PTE-1; hACTE-III; hACTEIII; hTE??[Similar Products]

The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. [provided by RefSeq, Oct 2010]

Acyl-coenzyme A (acyl-CoA) thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH (PubMed:9299485, PubMed:9153233, PubMed:15194431). Competes with bile acid CoA:amino acid N-acyltransferase (BAAT) for bile acid-CoA substrate (such as chenodeoxycholoyl-CoA). Shows a preference for medium-length fatty acyl-CoAs (C2 to C20) (PubMed:9299485, PubMed:9153233). Inactive towards substrates with more than C20 aliphatic chains (PubMed:9153233). Involved in the metabolic regulation of peroxisome proliferation (PubMed:15194431).

Jones JM., et al. (1999) J Biol Chem. 274 (14): 9216-9223.; Liu LX., et al. (1997) J Biol Chem. 272 (21): 13779-13785.

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