Full Product Name
ARF6, ID (ADP-ribosylation Factor 6)
Product Synonym Names
Anti -ARF6, ID (ADP-ribosylation Factor 6)
Product Gene Name
anti-ARF6 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 14; NC_000014.8 (50359736..50363772). Location: 14q21.3
3D Structure
ModBase 3D Structure for Q6FGZ2
Specificity
Recognizes human ADP-Ribosylation Factor 6 at ~19-20kD.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Immunogen
Recombinant human ARF6 protein.
Positive Control
NIH/3T3 Cell Lysate
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Other Notes
Small volumes of anti-ARF6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ARF6 antibody
ADP-Ribosylation Factor 6 (ARF6) is a GTP-binding protein that functions as a regulator of cytoskeleton remodeling and endocytic recycling. ARF6 is an allosteric activator of cholera toxin catalytic subunit, an ADP-ribosyltransferase. It may also be involved in the modulation of vesicle budding and uncoating with the Golgi apparatus.
Product Categories/Family for anti-ARF6 antibody
Antibodies; Abs to GTPase, Rab, Ras, Rho Proteins
Applications Tested/Suitable for anti-ARF6 antibody
Western Blot (WB)
Application Notes for anti-ARF6 antibody
Suitable for use in Western Blot.
Dilution: Western Blot: 1:500
NCBI/Uniprot data below describe general gene information for ARF6. It may not necessarily be applicable to this product.
UniProt Primary Accession #
Q6FGZ2
[Other Products]
UniProt Related Accession #
P62330; Q5U025; Q6FGZ2; Q6FH17[Other Products]
Molecular Weight
20,082 Da[Similar Products]
NCBI Official Full Name
ARF6
NCBI Official Synonym Full Names
ADP-ribosylation factor 6
NCBI Official Symbol
ARF6??[Similar Products]
NCBI Official Synonym Symbols
DKFZp564M0264
??[Similar Products]
NCBI Protein Information
ADP-ribosylation factor 6; OTTHUMP00000178980
UniProt Protein Name
ADP-ribosylation factor 6
Protein Family
Auxin response factor
UniProt Gene Name
ARF6??[Similar Products]
UniProt Entry Name
Q6FGZ2_HUMAN
NCBI Summary for ARF6
This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7. [provided by RefSeq]
UniProt Comments for ARF6
ARF6: GTP-binding protein involved in protein trafficking; regulates endocytic recycling and cytoskeleton remodeling. May modulate vesicle budding and uncoating within the Golgi apparatus. Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in the regulation of dendritic spine development. Contributes to the regulation of dendritic branching and filopodia extension. Interacts with ARHGAP21, ASAP2, HERC1, PIP5K1C and UACA. Interacts with NCS1/FREQ at the plasma membrane. Interacts with RAB11FIP3 and RAB11FIP4. Interacts with USP6 (via Rab-GAP TBC domain). Interacts with ECM29. Interacts with TBC1D24. Belongs to the small GTPase superfamily. Arf family.
Protein type: Motility/polarity/chemotaxis; G protein, monomeric; G protein, monomeric, ARF
Chromosomal Location of Human Ortholog: 14q21.3
Cellular Component: Golgi apparatus; ruffle; filopodium membrane; focal adhesion; membrane; endocytic vesicle; recycling endosome membrane; early endosome; plasma membrane; cell cortex; midbody; endosome; cleavage furrow
Molecular Function: GTPase activity; protein binding; GTP binding; thioesterase binding
Biological Process: myeloid cell apoptosis; metabolic process; regulation of filopodium formation; cell cycle; liver development; vesicle-mediated transport; regulation of Rac protein signal transduction; protein transport; positive regulation of actin filament polymerization; cell division; small GTPase mediated signal transduction; ruffle organization and biogenesis; negative regulation of receptor-mediated endocytosis; cortical actin cytoskeleton organization and biogenesis; cell adhesion; cell motility
Research Articles on ARF6
1. In the group of squamous cell lung cancer Arf6 expression elevation was observed more often.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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