Full Product Name
Anti-ARF6 Antibody
Product Gene Name
anti-ARF6 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Species Reactivity
Human, Mouse, Rat
Specificity
Western blot analysis of extracts of various cell lines, using ARF6 antibody. Immunohistochemistry of paraffin-embedded human breast cancer tissue using ARF6 antibody. Immunofluorescence analysis of A549 cell using ARF6 antibody. Blue: DAPI for nuclear staining.
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Immunogen
A synthetic peptide of human ARF6
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol, pH7.3.
Preparation and Storage
Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.
Other Notes
Small volumes of anti-ARF6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ARF6 antibody
This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7.
Applications Tested/Suitable for anti-ARF6 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-ARF6 antibody
WB: 1:500 - 1:2000
IHC: 1:50- 1:100
IF: 1:50- 1:100
Immunohistochemistry (IHC) of anti-ARF6 antibody
NCBI/Uniprot data below describe general gene information for ARF6. It may not necessarily be applicable to this product.
NCBI Accession #
CAG46762.1
[Other Products]
UniProt Secondary Accession #
P26438; Q6FGZ2[Other Products]
UniProt Related Accession #
P62330[Other Products]
Molecular Weight
20,082 Da
NCBI Official Full Name
ARF6, partial
NCBI Official Synonym Full Names
ADP ribosylation factor 6
NCBI Official Symbol
ARF6??[Similar Products]
NCBI Protein Information
ADP-ribosylation factor 6
UniProt Protein Name
ADP-ribosylation factor 6
Protein Family
Auxin response factor
UniProt Gene Name
ARF6??[Similar Products]
NCBI Summary for ARF6
This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7. [provided by RefSeq, Jul 2008]
UniProt Comments for ARF6
ARF6: GTP-binding protein involved in protein trafficking; regulates endocytic recycling and cytoskeleton remodeling. May modulate vesicle budding and uncoating within the Golgi apparatus. Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in the regulation of dendritic spine development. Contributes to the regulation of dendritic branching and filopodia extension. Interacts with ARHGAP21, ASAP2, HERC1, PIP5K1C and UACA. Interacts with NCS1/FREQ at the plasma membrane. Interacts with RAB11FIP3 and RAB11FIP4. Interacts with USP6 (via Rab-GAP TBC domain). Interacts with ECM29. Interacts with TBC1D24. Belongs to the small GTPase superfamily. Arf family.
Protein type: G protein, monomeric; G protein, monomeric, ARF; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 14q21.3
Cellular Component: cell cortex; endocytic vesicle; endosome; filopodium membrane; focal adhesion; membrane; plasma membrane; recycling endosome membrane; ruffle
Molecular Function: GTP binding; GTPase activity; protein binding; thioesterase binding
Biological Process: cell adhesion; cell motility; cortical actin cytoskeleton organization and biogenesis; negative regulation of receptor-mediated endocytosis; positive regulation of actin filament polymerization; regulation of filopodium formation; regulation of Rac protein signal transduction; ruffle organization and biogenesis; vesicle-mediated transport
Research Articles on ARF6
1. The Squamous cell carcinoma of the tongue cell line SCC-9, which overexpress Arf6 and AMAP1, also expressed EPB41L5 at high levels to promote invasiveness, whereas the weakly invasive SCC-25 cells did not express EPB41L5 at notable levels.
Precautions
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Disclaimer
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