MNX1 (Motor neuron and pancreas homeobox protein 1)/HLXB9/Homeobox protein HB9

For Research Use Only. Not for use in diagnostic procedures.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

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Small volumes of MNX1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS765678 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Motor neuron and pancreas homeobox protein 1 (MNX1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MNX1. The ELISA analytical biochemical technique of the MBS765678 kit is based on MNX1 antibody-MNX1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MNX1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MNX1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-MNX1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MNX1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MNX1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MNX1 can be calculated.

20,553 Da

motor neuron and pancreas homeobox 1

motor neuron and pancreas homeobox protein 1

Motor neuron and pancreas homeobox protein 1

HLXB9??[Similar Products]

MNX1_HUMAN

This gene encodes a nuclear protein, which contains a homeobox domain and is a transcription factor. Mutations in this gene result in Currarino syndrome, an autosomic dominant congenital malformation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

MNX1: Putative transcription factor involved in pancreas development and function. Defects in MNX1 are a cause of Currarino syndrome (CURRAS). The triad of a presacral tumor, sacral agenesis and anorectal malformation constitutes the Currarino syndrome which is caused by dorsal-ventral patterning defects during embryonic development. The syndrome occurs in the majority of patients as an autosomal dominant trait.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 7q36

Cellular Component: cytoplasm; nucleolus; nucleus

Molecular Function: sequence-specific DNA binding; transcription factor activity

Biological Process: anatomical structure morphogenesis; dorsoventral neural tube patterning; endocrine pancreas development; humoral immune response; motor axon guidance; nerve development; neurite morphogenesis; neuron migration; post-embryonic development; regulation of transcription from RNA polymerase II promoter; spinal cord motor neuron cell fate specification; transcription, DNA-dependent

Disease: Currarino Syndrome

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