Product Name
AMTN, Recombinant Protein
Full Product Name
AMTN, 17-209aa, Human, His tag, E Coli
Product Synonym Names
amelotin; uNQ689
Product Gene Name
AMTN recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
MGSSHHHHHH SSGLVPRGSH MGSLPQLKPA LGLPPTKLAP DQGTLPNQQQ SNQVFPSLSL IPLTQMLTLG PDLHLLNPAA GMTPGTQTHP LTLGGLNVQQ QLHPHVLPIF VTQLGAQGTI LSSEELPQIF TSLIIHSLFP GGILPTSQAG ANPDVQDGSL PAGGAGVNPA TQGTPAGRLP TPSGTDDDFA VTTPAGIQRS THAIEEATTE SANGIQ
3D Structure
ModBase 3D Structure for Q6UX39
Purity/Purification
90>% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol, 2mM DTT
Concentration
0.5 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of AMTN recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
AMTN recombinant protein
AMTN belongs to the amelotin family. It could be a cell adhesion protein involved in the maturation of tooth enamel. AMTN is a recently discovered secreted enamel protein predominantly expressed during the maturation stage of enamel formation. It accumulates in a basal lamina-like structure at the interface between ameloblasts and enamel mineral and it co-localizes with another recently described enamel protein, odontogenic ameloblast-associated protein (ODAM). Recombinant human AMTN protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for AMTN recombinant protein
Signal Transduction
Applications Tested/Suitable for AMTN recombinant protein
SDS-PAGE
SDS-Page of AMTN recombinant protein
NCBI/Uniprot data below describe general gene information for AMTN. It may not necessarily be applicable to this product.
NCBI Accession #
NP_997722
[Other Products]
NCBI GenBank Nucleotide #
NM_212557.3
[Other Products]
UniProt Primary Accession #
Q6UX39
[Other Products]
UniProt Secondary Accession #
Q0P503; Q0P506[Other Products]
UniProt Related Accession #
Q6UX39[Other Products]
Molecular Weight
22.2kDa (216aa) confirmed by MALDI-TOF
NCBI Official Full Name
amelotin isoform 1
NCBI Official Synonym Full Names
amelotin
NCBI Official Symbol
AMTN??[Similar Products]
NCBI Official Synonym Symbols
AI3B; UNQ689
??[Similar Products]
NCBI Protein Information
amelotin
UniProt Protein Name
Amelotin
UniProt Gene Name
AMTN??[Similar Products]
NCBI Summary for AMTN
The mineralized portions of teeth, the dentin and enamel, are formed by mesenchyme-derived odontoblasts and epithelium-derived ameloblasts, respectively. As ameloblasts differentiate, they deposit specific proteins necessary for enamel formation, including amelogenin (AMELX; MIM 300391), enamelin (ENAM; MIM 606585), and ameloblastin (AMBN; MIM 601259), in the organic enamel matrix. Amelotin is specifically expressed in maturation-stage ameloblasts (Iwasaki et al., 2005 [PubMed 16304441]).[supplied by OMIM, Mar 2008]
UniProt Comments for AMTN
Is a promoter of calcium phosphate mineralization, playing a critical role in the formation of the compact, mineralized, aprismatic enamel surface layer during the maturation stage of amelogenesis.
Product References and Citations for AMTN recombinant protein
Holcroft J, et al. (2011) Eur J Oral Sci. Dec;119 Suppl 1:301-6.
Research Articles on AMTN
1. Results show that AMTN and KLK4 are not essential for
biological processes outside of the dentition or during the secretory stage of amelogenesis. Both KLK4 and AMTN proved to be essential for the maturation of dental enamel, a process that requires the removal of extracellularmatrix proteins and the deposition of ions on the sides of enamel crystallites.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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