Full Product Name
IHH Polyclonal Antibody
Product Synonym Names
IHH; Ihh ; IHH; BDA1; HHG2; Indian hedgehog protein C-product; HHG-2;
Product Gene Name
anti-IHH antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 2; NC_000002.11 (219919142..219925238, complement). Location: 2q33-q35
3D Structure
ModBase 3D Structure for Q14623
Species Reactivity
Human, mouse, rat
Purity/Purification
Affinity purification
Immunogen
Fusion protein of human IHH
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at-20 degree C or-80 degree C. Avoid freeze / thaw cycles.
Other Notes
Small volumes of anti-IHH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-IHH antibody
Hedgehog proteins (Hh) are secreted signaling proteins that play many roles during animal development. Aberrant Hh signaling activity can be associated with numerous birth defects and uncontrolled Hh pathway activation is linked to the development of several types of cancers (1-2). The three identified vertebrate Hh genes are Sonic (Shh), Indian (Ihh) and Desert (Dhh), all of which have distinct as well as overlapping roles (3-5). Hh proteins are synthesized as 45 kDa precursors that undergo auto-cleavage to generate a 19 kDa amino-terminal peptide (Hh-N) and a carboxy-terminal peptide (Hh-C). The amino-terminal peptide becomes covalently attached to a cholesterol molecule at its carboxy terminus and acetylated at its amino terminus. This doubly modified Hh-N peptide is released from cells and responsible for all known Hedgehog signaling activity (6).
Applications Tested/Suitable for anti-IHH antibody
Western Blot (WB)
Application Notes for anti-IHH antibody
Western Blot (WB): 1:500-1:1000
Western Blot (WB) of anti-IHH antibody
Western blot analysis of extracts of Heart cells, using IHH antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
NCBI/Uniprot data below describe general gene information for IHH. It may not necessarily be applicable to this product.
NCBI Accession #
NP_002172.2
[Other Products]
NCBI GenBank Nucleotide #
NM_002181.3
[Other Products]
UniProt Primary Accession #
Q14623
[Other Products]
UniProt Secondary Accession #
O43322; Q8N4B9; B9EGM5[Other Products]
UniProt Related Accession #
Q14623[Other Products]
Molecular Weight
45kDa[Similar Products]
NCBI Official Full Name
indian hedgehog protein
NCBI Official Synonym Full Names
indian hedgehog
NCBI Official Symbol
IHH??[Similar Products]
NCBI Official Synonym Symbols
BDA1; HHG2
??[Similar Products]
NCBI Protein Information
indian hedgehog protein; HHG-2; Indian hedgehog homolog
UniProt Protein Name
Indian hedgehog protein
UniProt Synonym Protein Names
HHG-2
Protein Family
Indian hedgehog protein
UniProt Gene Name
IHH??[Similar Products]
UniProt Synonym Gene Names
IHH??[Similar Products]
UniProt Entry Name
IHH_HUMAN
Product References and Citations for anti-IHH antibody
1. Ingham, P.W. and McMahon, A.P. (2001) Genes Dev. 15, 3059-3087. 2. McMahon, A.P. et al. (2003) Curr. Top Dev. Biol. 53, 1-114. 3. Zhang, X.M. et al. (2001) Cell 106, 781-792. 4. Adolphe, C. et al. (2004) Development 131, 5009-5019. 5. Pathi, S. et al. (2001) Mech. Dev. 106, 107-117. 6. Bijlsma, M.F. et al. (2004) Bioessays 26, 387-394.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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