Full Product Name
IHH siRNA (Human)
Product Synonym Names
Indian hedgehog protein; IHH; HHG-2
Product Gene Name
IHH sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q14623
Specificity
IHH siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human IHH gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of IHH sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
IHH sirna
siRNA to inhibit IHH expression using RNA interference
Applications Tested/Suitable for IHH sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for IHH. It may not necessarily be applicable to this product.
NCBI Accession #
NP_002172.2
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NCBI GenBank Nucleotide #
NM_002181.3
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UniProt Primary Accession #
Q14623
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UniProt Secondary Accession #
O43322; Q8N4B9; B9EGM5[Other Products]
UniProt Related Accession #
Q14623[Other Products]
Molecular Weight
45,251 Da
NCBI Official Full Name
indian hedgehog protein
NCBI Official Synonym Full Names
indian hedgehog
NCBI Official Symbol
IHH??[Similar Products]
NCBI Official Synonym Symbols
BDA1; HHG2
??[Similar Products]
NCBI Protein Information
indian hedgehog protein
UniProt Protein Name
Indian hedgehog protein
UniProt Synonym Protein Names
HHG-2
Protein Family
Indian hedgehog protein
UniProt Gene Name
IHH??[Similar Products]
UniProt Synonym Gene Names
IHH??[Similar Products]
UniProt Entry Name
IHH_HUMAN
NCBI Summary for IHH
This gene encodes a member of the hedgehog family of secreted signaling molecules. Hedgehog proteins are essential regulators of a variety of developmental processes including growth, patterning and morphogenesis. The encoded protein specifically plays a role in bone growth an differentiation. Mutations in this gene are the cause of brachydactyly type A1 which is characterized by shortening or malformation of the phalanges. Mutations in this gene are also the cause of acrocapitofemoral dysplasia. [provided by RefSeq, Feb 2010]
UniProt Comments for IHH
IHH: Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP). Expressed in embryonic lung, and in ***** kidney and liver. Belongs to the hedgehog family.
Chromosomal Location of Human Ortholog: 2q33-q35
Cellular Component: extracellular matrix; extracellular space; plasma membrane
Molecular Function: peptidase activity; patched binding; calcium ion binding
Biological Process: vitelline membrane formation; proteoglycan metabolic process; maternal process involved in pregnancy; cell maturation; positive regulation of collagen biosynthetic process; cell fate specification; proteolysis; embryonic pattern specification; response to estradiol stimulus; positive regulation of T cell differentiation in the thymus; smooth muscle development; cell-cell signaling; embryonic digestive tract morphogenesis; regulation of growth; positive regulation of mesenchymal cell proliferation; pancreas development; positive regulation of smoothened signaling pathway; heart looping; skeletal development; bone resorption; negative regulation of immature T cell proliferation in the thymus; smoothened signaling pathway; intein-mediated protein splicing; in utero embryonic development; multicellular organism growth; negative regulation of eye pigmentation; negative regulation of T cell differentiation in the thymus; negative regulation of signal transduction; patterning of blood vessels; osteoblast differentiation; positive regulation of chondrocyte differentiation; cartilage development; neuron development; positive regulation of transcription from RNA polymerase II promoter; embryonic camera-type eye morphogenesis; embryonic digit morphogenesis; positive regulation of epithelial cell proliferation; positive regulation of alpha-beta T cell differentiation; negative regulation of apoptosis; negative regulation of alpha-beta T cell differentiation
Disease: Brachydactyly, Type A1; Acrocapitofemoral Dysplasia
Research Articles on IHH
1. Gene transfer of indian hedgehog protein results in chondrogenic induction of mesenchymal stem cells.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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