Product Name
Megakaryocyte Potentiating Factor (MSLN), Monoclonal Antibody
Popular Item
Full Product Name
Megakaryocyte Potentiating Factor (MPF)
Product Synonym Names
Anti -Megakaryocyte Potentiating Factor (MPF)
Product Gene Name
anti-MSLN antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 16; NC_000016.9 (810765..818865). Location: 16p13.3
3D Structure
ModBase 3D Structure for Q13421
Specificity
Recognizes human MPF.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 200ul sterile 40-50% glycerol, PBS.
Concentration
~0.5 mg/ml (after reconstitution) (lot specific)
Immunogen
Recombinant protein corresponding to aa35-289 from human MPF expressed in NS0 cells(Q13421)
Hybridoma
Myeloma cells with B cells with purified, NS0-derived, recombinant human CD27 Ligand (rhCD27 Ligand; Accession #NP_001243) extracellular domain.
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute with sterile 40-50% glycerol, PBS. Aliquot and store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-MSLN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-MSLN antibody
Megakaryocyte Potentiating Factor (MPF) is a 33kD soluble protein that is cleaved as an N-terminal propeptide from the mouse mesothelin preproprotein by furin, while the C-terminal portion is processed further to form mesothelin. Mouse MPF potentiates megakaryocyte colony forming activity of IL-3 in mouse bone marrow culture. Human MPF shows 55% amino acid identity with mouse or rat MPF. The preproprotein is produced by mesothelial cells and is also highly expressed in pancreatic, gastric and ovarian carcinomas.
Product Categories/Family for anti-MSLN antibody
Antibodies; Abs to Proteins
Applications Tested/Suitable for anti-MSLN antibody
Direct ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-MSLN antibody
Dilution: Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
NCBI/Uniprot data below describe general gene information for MSLN. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001170826.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001177355.1
[Other Products]
UniProt Primary Accession #
Q13421
[Other Products]
UniProt Secondary Accession #
Q14859; Q4VQD5; Q96GR6; Q96KJ5; Q9BR17; Q9BTR2; Q9UCB2; Q9UK57; D3DU65[Other Products]
UniProt Related Accession #
Q13421[Other Products]
Molecular Weight
68,986 Da[Similar Products]
NCBI Official Full Name
mesothelin isoform 1 preproprotein
NCBI Official Synonym Full Names
mesothelin
NCBI Official Symbol
MSLN??[Similar Products]
NCBI Official Synonym Symbols
MPF; SMRP
??[Similar Products]
NCBI Protein Information
mesothelin; CAK1 antigen; megakaryocyte potentiating factor; soluble MPF mesothelin related protein; pre-pro-megakaryocyte-potentiating factor
UniProt Protein Name
Mesothelin
UniProt Synonym Protein Names
CAK1 antigen; Pre-pro-megakaryocyte-potentiating factor
Protein Family
Mesothelin
UniProt Gene Name
MSLN??[Similar Products]
UniProt Synonym Gene Names
MPF; MPF??[Similar Products]
UniProt Entry Name
MSLN_HUMAN
NCBI Summary for MSLN
This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010]
UniProt Comments for MSLN
MSLN: Membrane-anchored forms may play a role in cellular adhesion. Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer. Belongs to the mesothelin family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, GPI anchor; Cell adhesion
Chromosomal Location of Human Ortholog: 16p13.3
Cellular Component: Golgi apparatus; extracellular space; cell surface; membrane; plasma membrane
Molecular Function: protein binding
Biological Process: pancreas development; cell adhesion
Research Articles on MSLN
1. Data indicate that binding of mesothelin to CA125 does not alter the measurement of mesothelin for the detection of malignant mesothelioma (MM).
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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