Product Name
3/8/2017, Polyclonal Antibody
Full Product Name
MARCH8 Antibody
Product Synonym Names
MARCH8; C-MIR; RING finger protein 178; RNF178; MARCH-VIII; MIR
Product Gene Name
anti-3/8/2017 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q5T0T0
Specificity
MARCH8 Antibody detects endogenous levels of total 42437
Form/Format
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0mg/mL (lot specific)
Immunogen
A synthesized peptide derived from human MARCH8
Preparation and Storage
Store at -20 degree C
Other Notes
Small volumes of anti-3/8/2017 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-3/8/2017 antibody
Total protein Ab
Applications Tested/Suitable for anti-3/8/2017 antibody
Western Blot (WB)
Application Notes for anti-3/8/2017 antibody
Western Blot: 1:1000-3000
Western Blot (WB) of anti-3/8/2017 antibody
Western blot analysis MARCH8 using HuvEc whole cell lysates
NCBI/Uniprot data below describe general gene information for 3/8/2017. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001002266.1
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NCBI GenBank Nucleotide #
NM_001002266.2
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UniProt Primary Accession #
Q5T0T0
[Other Products]
UniProt Secondary Accession #
Q5T0S8; Q8TC72; B2R8E7; H0Y7C6[Other Products]
UniProt Related Accession #
Q5T0T0[Other Products]
Molecular Weight
63,319 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase MARCH8 isoform a
NCBI Official Synonym Full Names
membrane associated ring-CH-type finger 8
NCBI Official Symbol
MARCH8??[Similar Products]
NCBI Official Synonym Symbols
MIR; CMIR; c-MIR; RNF178; MARCH-VIII
??[Similar Products]
NCBI Protein Information
E3 ubiquitin-protein ligase MARCH8
UniProt Protein Name
E3 ubiquitin-protein ligase MARCH8
UniProt Synonym Protein Names
Cellular modulator of immune recognition; c-MIR; Membrane-associated RING finger protein 8; Membrane-associated RING-CH protein VIII; MARCH-VIII; RING finger protein 178
UniProt Gene Name
MARCH8??[Similar Products]
UniProt Synonym Gene Names
MIR; RNF178; c-MIR; MARCH-VIII??[Similar Products]
UniProt Entry Name
MARH8_HUMAN
NCBI Summary for 3/8/2017
MARCH8 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH8 induces the internalization of several membrane glycoproteins (Goto et al., 2003 [PubMed 12582153]; Bartee et al., 2004 [PubMed 14722266]).[supplied by OMIM, Apr 2010]
UniProt Comments for 3/8/2017
MARCH8: E3 ubiquitin-protein ligase that mediates ubiquitination of CD86 and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. May also promote ubiquitination and endocytosis of TFRC and FAS.
Protein type: EC 6.3.2.-; EC 6.3.2.19; Ligase; Membrane protein, integral; Membrane protein, multi-pass; Ubiquitin conjugating system; Ubiquitin ligase
Chromosomal Location of Human Ortholog: 10q11.21
Cellular Component: endosome; lysosome
Molecular Function: ubiquitin-protein ligase activity
Biological Process: protein polyubiquitination
Research Articles on 3/8/2017
1. MARCH8 is highly expressed in terminally differentiated myeloid cells, and that it is a potent antiviral protein that targets viral envelope glycoproteins and reduces their incorporation into virions.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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