cGMP-specific 3'; 5'-cyclic phosphodiesterase; cGMP-binding cGMP-specific phosphodiesterase; CGB-PDE; Pde5a; Pde5; 3.1.4.35

For Research Use Only. Not for use in diagnostic procedures.

Store at 4 degree C

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Small volumes of Pde5a elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9425613 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cGMP-specific 3', 5'-cyclic phosphodiesterase (Pde5a) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Pde5a. The ELISA analytical biochemical technique of the MBS9425613 kit is based on Pde5a antibody-Pde5a antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Pde5a antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Pde5a. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

a?? Da

phosphodiesterase 5A

cGMP-specific 3',5'-cyclic phosphodiesterase

cGMP-specific 3',5'-cyclic phosphodiesterase

Pde5; CGB-PDE??[Similar Products]

catalyzes the hydrolysis of cGMP to 5'-GMP; may regulate vascular relaxation; may be involved in establishment of cerebellar neural networks [RGD, Feb 2006]

PDE5A: a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Phosphorylation is regulated by binding of cGMP to the two allosteric sites. Sildenafil (Viagra) is a highly selective and potent inhibitor of PDE5A. Alternative splicing results in four transcript variants encoding distinct isoforms.

Protein type: EC 3.1.4.35; Nucleotide Metabolism - purine; Phosphodiesterase

Chromosomal Location of Human Ortholog: 2q42

Cellular Component: cytosol

Molecular Function: 3',5'-cyclic-GMP phosphodiesterase activity; 3',5'-cyclic-nucleotide phosphodiesterase activity; cGMP binding; cyclic-nucleotide phosphodiesterase activity

Biological Process: cGMP catabolic process; cGMP metabolic process; negative regulation of T cell proliferation; nervous system development; positive regulation of apoptosis; positive regulation of chronic inflammatory response; positive regulation of MAP kinase activity; positive regulation of vasoconstriction; regulation of cGMP metabolic process; regulation of the force of heart contraction; response to hypoxia; response to lipopolysaccharide; response to testosterone stimulus; short-term memory; vasodilation

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