cGMP-specific 3'; 5'-cyclic phosphodiesterase; cGMP-binding cGMP-specific phosphodiesterase; CGB-PDE; PDE5A; PDE5; 3.1.4.35

For Research Use Only. Not for use in diagnostic procedures.

For long term storage, please store the entire kit at -20 degree C.

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Small volumes of PDE5A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2887211 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cGMP-specific 3',5'-cyclic phosphodiesterase (PDE5A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PDE5A. The ELISA analytical biochemical technique of the MBS2887211 kit is based on PDE5A antibody-PDE5A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PDE5A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PDE5A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

94,805 Da

phosphodiesterase 5A

cGMP-specific 3',5'-cyclic phosphodiesterase

cGMP-specific 3',5'-cyclic phosphodiesterase

PDE5; CGB-PDE??[Similar Products]

PDE5A_HUMAN

This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

PDE5A: a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Phosphorylation is regulated by binding of cGMP to the two allosteric sites. Sildenafil (Viagra) is a highly selective and potent inhibitor of PDE5A. Alternative splicing results in four transcript variants encoding distinct isoforms.

Protein type: Phosphodiesterase; EC 3.1.4.35; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 4q27

Cellular Component: cytosol

Molecular Function: 3',5'-cyclic-GMP phosphodiesterase activity; 3',5'-cyclic-nucleotide phosphodiesterase activity; cGMP binding; metal ion binding

Biological Process: blood coagulation; cGMP catabolic process; negative regulation of T cell proliferation; positive regulation of MAP kinase activity; regulation of cGMP metabolic process; signal transduction

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