For Research Use Only. Not for use in diagnostic procedures.

This assay recognizes recombinant and natural mouse PDE5A. No significant cross-reactivity or interference was observed.

For long term storage, please store the entire kit at -20 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of Pde5a elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS288092 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cGMP-specific 3',5'-cyclic phosphodiesterase (Pde5a) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Pde5a. The ELISA analytical biochemical technique of the MBS288092 kit is based on Pde5a antibody-Pde5a antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Pde5a antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Pde5a. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PDE5A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PDE5A and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PDE5A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PDE5A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

98,407 Da

phosphodiesterase 5A, cGMP-specific

cGMP-specific 3',5'-cyclic phosphodiesterase

cGMP-specific 3',5'-cyclic phosphodiesterase

Pde5; CGB-PDE??[Similar Products]

PDE5A_MOUSE

PDE5A: a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Phosphorylation is regulated by binding of cGMP to the two allosteric sites. Sildenafil (Viagra) is a highly selective and potent inhibitor of PDE5A. Alternative splicing results in four transcript variants encoding distinct isoforms.

Protein type: Nucleotide Metabolism - purine; EC 3.1.4.35; Phosphodiesterase

Cellular Component: cytosol

Molecular Function: 3',5'-cyclic-GMP phosphodiesterase activity; 3',5'-cyclic-nucleotide phosphodiesterase activity; catalytic activity; cGMP binding; cyclic-nucleotide phosphodiesterase activity; hydrolase activity; metal ion binding; nucleotide binding; phosphoric diester hydrolase activity

Biological Process: cGMP catabolic process; cGMP metabolic process; metabolic process; negative regulation of T cell proliferation; positive regulation of apoptosis; positive regulation of chronic inflammatory response; positive regulation of G-protein coupled receptor protein signaling pathway; positive regulation of MAP kinase activity; positive regulation of vasoconstriction; regulation of cGMP metabolic process; regulation of the force of heart contraction; short-term memory; signal transduction

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