AMCBX1; FIP 3; FIP3; FIP-3; FIP3; NEMO; I kappa B kinase gamma; I-kappa-B kinase subunit gamma; IkB kinase gamma subunit; IkB kinase subunit gamma; IkB kinase-associated protein 1; IKBKG; IKK gamma; IKKAP 1; IKKAP1; IKKG; IKKgamma; IKK-gamma; Incontinentia pigmenti; IP2; IPD2; NF-kappa-B essential modifier; NF-kappa-B essential modulator; NFkappaB essential modulator(NEMO); Q9Y6K9; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Immunogen affinity purified.

Lyophilized
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Small volumes of anti-IKBKG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Rabbit IgG polyclonal antibody for NF-kappa-B essential modulator(IKBKG) detection.
Background: NF-kappa-B essential modulator (NEMO), also known as inhibitor of nuclear factor kappa-B kinase subunit gamma (IKK-gamma), is a protein that in humans is encoded by the IKBKG gene. This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome.

Western Blot (WB)

Western Blot: 0.1-0.5ug/ml

Figure 1. Western blot analysis of IKK gamma using anti-IKK gamma antibody (MBS178627).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
lane 1: rat cardiac muscle tissue lysates,
lane 2: HEPG2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKK gamma antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IKK gamma at approximately 48KD. The expected band size for IKK gamma is at 48KD.

36,953 Da

inhibitor of nuclear factor kappa B kinase subunit gamma

NF-kappa-B essential modulator

NF-kappa-B essential modulator

FIP3; NEMO; NEMO; IKKAP1; I-kappa-B kinase subunit gamma; IKK-gamma; IKKG; IkB kinase subunit gamma??[Similar Products]

This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome. [provided by RefSeq, Mar 2016]

IKKG: a regulatory subunit of the IKK-signalosome complex. Interacts preferentially with IKK-beta but also able to interact with IKK-alpha, IKAP, TAX, RIP and MAP3K14/NIK. Defects are the cause of familial incontinentia pigmenti type II (IP2).

Protein type: Adaptor/scaffold; Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: cytoplasm; cytosol; IkappaB kinase complex; intracellular; nucleus; spindle pole; ubiquitin ligase complex

Molecular Function: protein binding; protein domain specific binding; protein heterodimerization activity; protein homodimerization activity; signal transducer activity; ubiquitin protein ligase binding

Biological Process: activation of MAPK activity; activation of NF-kappaB transcription factor; apoptosis; establishment of vesicle localization; I-kappaB kinase/NF-kappaB cascade; immune response; inflammatory response; innate immune response; JNK cascade; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of transcription from RNA polymerase II promoter; response to DNA damage stimulus; response to virus; stimulatory C-type lectin receptor signaling pathway; stress-activated MAPK cascade; T cell receptor signaling pathway

Disease: Ectodermal Dysplasia, Anhidrotic, With Immunodeficiency, Osteopetrosis, And Lymphedema; Ectodermal Dysplasia, Hypohidrotic, With Immune Deficiency; Immunodeficiency 33; Immunodeficiency Without Anhidrotic Ectodermal Dysplasia; Incontinentia Pigmenti; Invasive Pneumococcal Disease, Recurrent Isolated, 2

1. Rothwarf, D. M., Zandi, E., Natoli, G., Karin, M.IKK-gamma is an essential regulatory subunit of the I-kappa-B kinase complex.Nature 395: 297-300, 1998.
2. Yamaoka, S., Courtois, G., Bessia, C., Whiteside, S. T., Weil, R., Agou, F., Kirk, H. E., Kay, R. J., Israel, A.Complementation cloning of NEMO, a component of the I-kappa-B kinase complex essential for NF-kappa-B activation.Cell 93: 1231-1240, 1998.

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