For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of PLCD1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7235656 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 1 phosphatidylinositol 4, 5 bisphosphate phosphodiesterase delta 1 (PLCD1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PLCD1. The ELISA analytical biochemical technique of the MBS7235656 kit is based on PLCD1 antibody-PLCD1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PLCD1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PLCD1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Signal Transduction

88,135 Da

phospholipase C, delta 1

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1; PLC-delta-1; phospholipase C-III; phosphoinositide phospholipase C-delta-1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase delta-1

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1

PLC-III; PLC-delta-1??[Similar Products]

PLCD1_HUMAN

This gene encodes a member of the phospholipase C family. Phospholipase C isozymes play critical roles in intracellular signal transduction by catalyzing the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) into the second messengers diacylglycerol (DAG) and inositol triphosphate (IP3). The encoded protein functions as a tumor suppressor in several types of cancer, and mutations in this gene are a cause of hereditary leukonychia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

PLCD1: The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Essential for trophoblast and placental development. Defects in PLCD1 are the cause of nail disorder non- syndromic congenital type 3 (NDNC3). NDNC3 is a nail disorder characterized by a white appearance of the nail plate (true leukonychia), the nail bed (pseudoleukonychia), or neither (apparent leukonychia). Leukonychia may involve all of the nail (leukonychia totalis) or only part of the nail (leukonychia partialis), or can appear as one or more transverse bands (leukonychia striata) or white spots (leukonychia punctata). 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.4.11; Carbohydrate Metabolism - inositol phosphate; Phospholipase

Chromosomal Location of Human Ortholog: 3p22-p21.3

Cellular Component: cytoplasm; plasma membrane; cytosol

Molecular Function: signal transducer activity; phosphatidylserine binding; GTPase activating protein binding; calcium ion binding; phosphoinositide phospholipase C activity

Biological Process: inositol phosphate metabolic process; phospholipid metabolic process; angiogenesis; lipid catabolic process; regulation of cell proliferation

Disease: Nail Disorder, Nonsyndromic Congenital, 3

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