Product Name
MAN2B1, Polyclonal Antibody
Popular Item
Full Product Name
MAN2B1 Polyclonal Antibody
Product Gene Name
anti-MAN2B1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O00754
Purity/Purification
Affinity purification
Immunogen
Recombinant protein of human MAN2B1
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Other Notes
Small volumes of anti-MAN2B1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-MAN2B1 antibody
Polyclonal
Applications Tested/Suitable for anti-MAN2B1 antibody
Western Blot (WB)
Application Notes for anti-MAN2B1 antibody
WB: 1:200 - 1:500
Western Blot (WB) of anti-MAN2B1 antibody
Western blot analysis of extracts of mouse liver, using MAN2B1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
NCBI/Uniprot data below describe general gene information for MAN2B1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000519.2
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NCBI GenBank Nucleotide #
NM_000528.3
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UniProt Primary Accession #
O00754
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UniProt Secondary Accession #
O15330; Q16680; Q93094; Q9BW13; G5E928[Other Products]
UniProt Related Accession #
O00754[Other Products]
Molecular Weight
Calculated MW: 114kDa
Molecular Weight: 1011
NCBI Official Full Name
lysosomal alpha-mannosidase isoform 1
NCBI Official Synonym Full Names
mannosidase alpha class 2B member 1
NCBI Official Symbol
MAN2B1??[Similar Products]
NCBI Official Synonym Symbols
MANB; LAMAN
??[Similar Products]
NCBI Protein Information
lysosomal alpha-mannosidase
UniProt Protein Name
Lysosomal alpha-mannosidase
UniProt Synonym Protein Names
Lysosomal acid alpha-mannosidase; Mannosidase alpha class 2B member 1; Mannosidase alpha-B
UniProt Gene Name
MAN2B1??[Similar Products]
UniProt Synonym Gene Names
LAMAN; MANB; Laman??[Similar Products]
UniProt Entry Name
MA2B1_HUMAN
NCBI Summary for MAN2B1
This gene encodes an enzyme that hydrolyzes terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides. Its activity is necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover and it is member of family 38 of glycosyl hydrolases. The full length protein is processed in two steps. First, a 49 aa leader sequence is cleaved off and the remainder of the protein is processed into 3 peptides of 70 kDa, 42 kDa (D) and 13/15 kDa (E). Next, the 70 kDa peptide is further processed into three peptides (A, B and C). The A, B and C peptides are disulfide-linked. Defects in this gene have been associated with lysosomal alpha-mannosidosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010]
UniProt Comments for MAN2B1
MAN2B1: Necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover. Cleaves all known types of alpha-mannosidic linkages. Defects in MAN2B1 are the cause of lysosomal alpha- mannosidosis (AM). AM is a lysosomal storage disease characterized by accumulation of unbranched oligosaccharide chains. This accumulation is expressed histologically as cytoplasmic vacuolation predominantly in the CNS and parenchymatous organs. Depending on the clinical findings at the age of onset, a severe infantile (type I) and a mild juvenile (type II) form of alpha-mannosidosis are recognized. There is considerable variation in the clinical expression with mental retardation, recurrent infections, impaired hearing and Hurler- like skeletal changes being the most consistent abnormalities. Belongs to the glycosyl hydrolase 38 family.
Protein type: Hydrolase; Glycan Metabolism - other glycan degradation; EC 3.2.1.24
Chromosomal Location of Human Ortholog: 19p13.2
Cellular Component: extracellular space; lysosome
Molecular Function: alpha-mannosidase activity; mannose binding; zinc ion binding
Biological Process: learning and/or memory; mannose metabolic process; protein deglycosylation; protein modification process
Disease: Mannosidosis, Alpha B, Lysosomal
Research Articles on MAN2B1
1. Our results indicate a correlation between the MAN2B1 genotypes and the cognitive function, upper limb coordination, balance, FVC% and the storage of oligosaccharides in CSF.
Precautions
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