Product Name
CCDC6, siRNA
Full Product Name
CCDC6 siRNA (Human)
Product Synonym Names
D10S170; TST1; Coiled-coil domain-containing protein 6; Papillary thyroid carcinoma-encoded protein; Protein H4
Product Gene Name
CCDC6 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q16204
Specificity
CCDC6 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CCDC6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CCDC6 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CCDC6 sirna
siRNA to inhibit CCDC6 expression using RNA interference
Applications Tested/Suitable for CCDC6 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CCDC6. It may not necessarily be applicable to this product.
NCBI Accession #
NP_005427.2
[Other Products]
NCBI GenBank Nucleotide #
NM_005436.4
[Other Products]
UniProt Primary Accession #
Q16204
[Other Products]
UniProt Secondary Accession #
Q15250; Q6GSG7[Other Products]
UniProt Related Accession #
Q16204[Other Products]
Molecular Weight
53,291 Da
NCBI Official Full Name
coiled-coil domain-containing protein 6
NCBI Official Synonym Full Names
coiled-coil domain containing 6
NCBI Official Symbol
CCDC6??[Similar Products]
NCBI Official Synonym Symbols
H4; PTC; TPC; TST1; D10S170
??[Similar Products]
NCBI Protein Information
coiled-coil domain-containing protein 6
UniProt Protein Name
Coiled-coil domain-containing protein 6
UniProt Synonym Protein Names
Papillary thyroid carcinoma-encoded protein; Protein H4
Protein Family
Coiled-coil domain-containing protein
UniProt Gene Name
CCDC6??[Similar Products]
UniProt Synonym Gene Names
D10S170; TST1??[Similar Products]
UniProt Entry Name
CCDC6_HUMAN
NCBI Summary for CCDC6
This gene encodes a coiled-coil domain-containing protein. The encoded protein is ubiquitously expressed and may function as a tumor suppressor. A chromosomal rearrangement resulting in the expression of a fusion gene containing a portion of this gene and the intracellular kinase-encoding domain of the ret proto-oncogene is the cause of thyroid papillary carcinoma.[provided by RefSeq, Sep 2010]
UniProt Comments for CCDC6
CCDC6: Defects in CCDC6 are a cause of thyroid papillary carcinoma (TPC). TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. A chromosomal aberration involving CCDC6 is found in thyroid papillary carcinomas. Inversion inv(10)(q11.2;q21) generates the RET/CCDC6 (PTC1) oncogene.
Protein type: Cytoskeletal; Oncoprotein; Apoptosis
Chromosomal Location of Human Ortholog: 10q21
Cellular Component: cytoskeleton; cytoplasm
Molecular Function: protein binding; structural constituent of cytoskeleton; SH3 domain binding
Disease: Thyroid Carcinoma, Papillary
Research Articles on CCDC6
1. CCDC6 levels provide valuable insight for OS.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
