Product Name
CLEC4C, siRNA
Full Product Name
CLEC4C siRNA (Human)
Product Synonym Names
BDCA2; CLECSF11; CLECSF7; DLEC; HECL; C-type lectin domain family 4 member C; Blood dendritic cell antigen 2; BDCA-2; C-type lectin superfamily member 7; Dendritic lectin; CD antigen CD303
Product Gene Name
CLEC4C sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8WTT0
Specificity
CLEC4C siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CLEC4C gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CLEC4C sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CLEC4C sirna
siRNA to inhibit CLEC4C expression using RNA interference
Applications Tested/Suitable for CLEC4C sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CLEC4C. It may not necessarily be applicable to this product.
NCBI Accession #
NP_569708.1
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NCBI GenBank Nucleotide #
NM_130441.2
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UniProt Primary Accession #
Q8WTT0
[Other Products]
UniProt Secondary Accession #
Q3T1C3; Q6UXS8; Q8WXX8; D3DUU3[Other Products]
UniProt Related Accession #
Q8WTT0[Other Products]
Molecular Weight
21,498 Da
NCBI Official Full Name
C-type lectin domain family 4 member C isoform 1
NCBI Official Synonym Full Names
C-type lectin domain family 4, member C
NCBI Official Symbol
CLEC4C??[Similar Products]
NCBI Official Synonym Symbols
DLEC; HECL; BDCA2; CD303; CLECSF7; CLECSF11; PRO34150
??[Similar Products]
NCBI Protein Information
C-type lectin domain family 4 member C
UniProt Protein Name
C-type lectin domain family 4 member C
UniProt Synonym Protein Names
Blood dendritic cell antigen 2; BDCA-2; C-type lectin superfamily member 7; Dendritic lectin; CD_antigen: CD303
Protein Family
C-type lectin domain family
UniProt Gene Name
CLEC4C??[Similar Products]
UniProt Synonym Gene Names
BDCA2; CLECSF11; CLECSF7; DLEC; HECL; BDCA-2??[Similar Products]
UniProt Entry Name
CLC4C_HUMAN
NCBI Summary for CLEC4C
This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type 2 transmembrane protein may play a role in dendritic cell function. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for CLEC4C
CLEC4C: Involved in antigen-capturing. Target ligand into antigen processing and peptide-loading compartments for presentation to T-cells. May mediate potent inhibition of induction of IFN-alpha/beta expression in plasmacytoid dendritic cells. May act as a signaling receptor that activates protein- tyrosine kinases and mobilizes intracellular calcium. Does not seem to bind mannose. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Receptor, misc.; Membrane protein, integral
Chromosomal Location of Human Ortholog: 12p13.2-p12.3
Cellular Component: integral to membrane
Molecular Function: carbohydrate binding
Biological Process: innate immune response
Research Articles on CLEC4C
1. We show the crystal structures of a carbohydrate recognition domain (CRD) of human C-type lectin receptor blood dendritic cell antigen-2 (BDCA2).
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
