A-inhibin subunit|inhibin alpha subunit; INH-A

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Human INHA. No significant cross-reactivity or interference between Human INHA and analogues was observed.

Store at 2-8 degree C.

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Small volumes of INHA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS285724 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Inhibin (INHA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing INHA. The ELISA analytical biochemical technique of the MBS285724 kit is based on INHA antibody-INHA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect INHA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, INHA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate INHA in samples. An antibody specific for INHA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyINHA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for INHA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of INHA bound in the initial step. The color development is stopped and the intensity of the color is measured.

39,670 Da

inhibin alpha subunit

inhibin alpha chain

Inhibin alpha chain

This gene encodes a member of the TGF-beta (transforming growth factor-beta) superfamily of proteins. The encoded preproprotein is proteolytically processed to generate multiple peptide products, including the alpha subunit of the inhibin A and B protein complexes. These complexes negatively regulate follicle stimulating hormone secretion from the pituitary gland. Inhibins have also been implicated in regulating numerous cellular processes including cell proliferation, apoptosis, immune response and hormone secretion. Mutations in this gene may be associated with male infertility and premature ovarian failure in female human patients. [provided by RefSeq, Aug 2016]

INHA: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: cell soma; cytoplasm; extracellular region; photoreceptor inner segment; photoreceptor outer segment

Molecular Function: cytokine activity; growth factor activity; hormone activity; protein binding; protein heterodimerization activity; receptor binding; transforming growth factor beta receptor binding

Biological Process: BMP signaling pathway; cell cycle arrest; cell development; cell differentiation; cell surface receptor signaling pathway; cell-cell signaling; erythrocyte differentiation; hemoglobin biosynthetic process; male gonad development; negative regulation of B cell differentiation; negative regulation of cell cycle; negative regulation of follicle-stimulating hormone secretion; negative regulation of interferon-gamma biosynthetic process; negative regulation of macrophage differentiation; negative regulation of phosphorylation; nervous system development; ovarian follicle development; positive regulation of follicle-stimulating hormone secretion; regulation of apoptosis; regulation of cell cycle; regulation of cell proliferation; regulation of MAPK cascade; response to external stimulus; signal transduction; skeletal system development

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