Human Lysosomal alpha-mannosidase (MAN2B1) ELISA kit; LAMAN; MANB; lysosomal acid alpha-mannosidase; mannosidase; alpha B; lysosomal; mannosidase; alpha; class 2B; member 1

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of human MAN2B1. No significant cross-reactivity or interference between human MAN2B1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of MAN2B1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS926128 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the mannosidase, alpha, class 2B, member 1 (MAN2B1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MAN2B1. The ELISA analytical biochemical technique of the MBS926128 kit is based on MAN2B1 antibody-MAN2B1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MAN2B1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MAN2B1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MAN2B1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAN2B1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAN2B1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAN2B1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

113,744 Da

mannosidase, alpha, class 2B, member 1

lysosomal alpha-mannosidase; mannosidase alpha-B; mannosidase, alpha B, lysosomal; lysosomal acid alpha-mannosidase

Lysosomal alpha-mannosidase

LAMAN; MANB; Laman??[Similar Products]

MA2B1_HUMAN

This gene encodes an enzyme that hydrolyzes terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides. Its activity is necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover and it is member of family 38 of glycosyl hydrolases. The full length protein is processed in two steps. First, a 49 aa leader sequence is cleaved off and the remainder of the protein is processed into 3 peptides of 70 kDa, 42 kDa (D) and 13/15 kDa (E). Next, the 70 kDa peptide is further processed into three peptides (A, B and C). The A, B and C peptides are disulfide-linked. Defects in this gene have been associated with lysosomal alpha-mannosidosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010]

MAN2B1: Necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover. Cleaves all known types of alpha-mannosidic linkages. Defects in MAN2B1 are the cause of lysosomal alpha- mannosidosis (AM). AM is a lysosomal storage disease characterized by accumulation of unbranched oligosaccharide chains. This accumulation is expressed histologically as cytoplasmic vacuolation predominantly in the CNS and parenchymatous organs. Depending on the clinical findings at the age of onset, a severe infantile (type I) and a mild juvenile (type II) form of alpha-mannosidosis are recognized. There is considerable variation in the clinical expression with mental retardation, recurrent infections, impaired hearing and Hurler- like skeletal changes being the most consistent abnormalities. Belongs to the glycosyl hydrolase 38 family.

Protein type: Glycan Metabolism - other glycan degradation; Hydrolase; EC 3.2.1.24

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: lysosome

Molecular Function: zinc ion binding; carbohydrate binding; alpha-mannosidase activity

Biological Process: mannose metabolic process; protein deglycosylation; protein modification process

Disease: Mannosidosis, Alpha B, Lysosomal

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