Full Product Name
DNL4 Antibody
Product Synonym Names
DNA ligase 4; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4; LIG4
Product Gene Name
anti-DNL4 antibody
[Similar Products]
Product Synonym Gene Name
LIG4[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P49917
Specificity
DNL4 Antibody detects endogenous levels of total DNL4 protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (lot specific)
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human DNL4.
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-DNL4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-DNL4 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-DNL4 antibody
WB: 1:500~1:1000
ELISA: 1:20000
Western Blot (WB) of anti-DNL4 antibody
Western blot analysis of lysates from Jurkat cells, using DNL4 Antibody. The lane on the right is blocked with the synthesized peptide.
NCBI/Uniprot data below describe general gene information for DNL4. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001091738.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001098268.1
[Other Products]
UniProt Primary Accession #
P49917
[Other Products]
UniProt Secondary Accession #
Q8IY66; Q8TEU5[Other Products]
UniProt Related Accession #
P49917[Other Products]
NCBI Official Full Name
DNA ligase 4 isoform 1
NCBI Official Synonym Full Names
DNA ligase 4
NCBI Official Symbol
LIG4??[Similar Products]
NCBI Official Synonym Symbols
LIG4S
??[Similar Products]
NCBI Protein Information
DNA ligase 4
UniProt Protein Name
DNA ligase 4
UniProt Synonym Protein Names
DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4
UniProt Gene Name
LIG4??[Similar Products]
NCBI Summary for DNL4
The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
UniProt Comments for DNL4
Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
Research Articles on DNL4
1. This study shown that both ligase IV and XRCC4 may act in concert to modulate the development of glioma.
Precautions
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Disclaimer
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