DNA ligase 4; EC 6.5.1.1; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4; LIG4

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

The antibody detects endogenous levels of total DNL4 protein.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

1.0 mg/ml (lot specific)

Store at -20 degree C

Small volumes of anti-DNL4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.Wei Y.-F., Mol. Cell. Biol. 15:3206-3216(1995).Dunham A., Nature 428:522-528(2004).Robins P., J. Biol. Chem. 271:24257-24261(1996).

Total protein Ab

Western Blot (WB)

Western blotting: 1:500~1:3000

Western blot analysis of extracts from Jurkat cells, using DNL4 antibody.

103,971 Da

ligase IV, DNA, ATP-dependent

DNA ligase 4

DNA ligase 4

DNLI4_HUMAN

The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]

LIG4: a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. Defects in this gene are the cause of LIG4 syndrome.

Protein type: Ligase; EC 6.5.1.1

Chromosomal Location of Human Ortholog: 13q33-q34

Cellular Component: nucleoplasm; DNA ligase IV complex; focal adhesion; cytoplasm; plasma membrane; condensed chromosome; DNA-dependent protein kinase complex; nucleus

Molecular Function: protein C-terminus binding; protein binding; DNA binding; DNA ligase activity; DNA ligase (ATP) activity; metal ion binding; ATP binding; ligase activity

Biological Process: central nervous system development; viral reproduction; somatic stem cell maintenance; T cell differentiation in the thymus; chromosome organization and biogenesis; double-strand break repair via nonhomologous end joining; DNA ligation; positive regulation of fibroblast proliferation; double-strand break repair; DNA ligation during DNA repair; response to gamma radiation; DNA ligation during DNA recombination; negative regulation of neuron apoptosis; response to X-ray; pro-B cell differentiation; single strand break repair; positive regulation of neurogenesis; V(D)J recombination; immunoglobulin V(D)J recombination; in utero embryonic development; isotype switching; DNA repair; cell cycle; lagging strand elongation; cell proliferation; neuron apoptosis; cell division; T cell receptor V(D)J recombination; nucleotide-excision repair, DNA gap filling

Disease: Myeloma, Multiple; Lig4 Syndrome

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