3-phosphoinositide-dependent protein kinase 1; hPDK1; PDPK1; PDK1

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Antigen Affinity Purified

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Small volumes of anti-PDPK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Serine/threonine kinase which acts as a master kinase, phosphorylating and activating a subgroup of the AGC family of protein kinases. Its targets include: protein kinase B (PKB/AKT1, PKB/AKT2, PKB/AKT3), p70 ribosomal protein S6 kinase (RPS6KB1), p90 ribosomal protein S6 kinase (RPS6KA1, RPS6KA2 and RPS6KA3), cyclic AMP-dependent protein kinase (PRKACA), protein kinase C (PRKCD and PRKCZ), serum and glucocorticoid-inducible kinase (SGK1, SGK2 and SGK3), p21-activated kinase-1 (PAK1), protein kinase PKN (PKN1 and PKN2). Plays a central role in the transduction of signals from insulin by providing the activating phosphorylation to PKB/AKT1, thus propagating the signal to downstream targets controlling cell proliferation and survival, as well as glucose and amino acid uptake and storage. Negatively regulates the TGF-beta-induced signaling by: modulating the association of SMAD3 and SMAD7 with TGF-beta receptor, phosphorylating SMAD2, SMAD3, SMAD4 and SMAD7, preventing the nuclear translocation of SMAD3 and SMAD4 and the translocation of SMAD7 from the nucleus to the cytoplasm in response to TGF-beta. Activates PPARG transcriptional activity and promotes adipocyte differentiation. Activates the NF-kappa-B pathway via phosphorylation of IKKB. The tyrosine phosphorylated form is crucial for the regulation of focal adhesions by angiotensin II. Controls proliferation, survival, and growth of developing pancreatic cells. Participates in the regulation of Ca2+ entry and Ca2+-activated K+ channels of mast cells. Essential for the motility of vascular endothelial cells (ECs) and is involved in the regulation of their chemotaxis. Plays a critical role in cardiac homeostasis by serving as a dual effector for cell survival and beta-adrenergic response. Plays an important role during thymocyte development by regulating the expression of key nutrient receptors on the surface of pre-T cells and mediating Notch-induced cell growth and proliferative responses. Provides negative feedback inhibition to toll-like receptor-mediated NF-kappa-B activation in macrophages. Isoform 3 is catalytically inactive.

ELISA (EIA), Immunohistochemistry (IHC)

Recommended dilution: IHC:1:20-1:200

Immunohistochemistry of paraffin-embedded human thyroid using MBS1493695 at dilution 1:100

Immunohistochemistry of paraffin-embedded human kidney using MBS1493695 at dilution 1:100

Immunofluorescent analysis of MCF-7 cells using MBS1493695 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

50,838 Da

3-phosphoinositide dependent protein kinase 1

3-phosphoinositide-dependent protein kinase 1

3-phosphoinositide-dependent protein kinase 1

PDK1; hPDK1??[Similar Products]

PDPK1_HUMAN

PDK1: an AGC kinase of the PKB family that contains a PH domain. Involved in a wide variety of processes including cell proliferation, differentiation and apoptosis. Autophosphorylation in the activation loop is necessary for activity. Phosphorylates and activates kinases including Akt, PKC isozymes, p70 S6K and RSK. Three alternatively-spliced isoforms have been described.

Protein type: Protein kinase, AGC; Kinase, protein; Nuclear receptor co-regulator; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; AGC group; PKB family

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: cell projection; cytoplasm; cytoplasmic membrane-bound vesicle; cytosol; focal adhesion; intracellular; membrane; nucleoplasm; plasma membrane

Molecular Function: 3-phosphoinositide-dependent protein kinase activity; ATP binding; insulin receptor binding; phospholipase activator activity; phospholipase binding; protein binding; protein kinase binding; protein serine/threonine kinase activity

Biological Process: actin cytoskeleton organization and biogenesis; activation of protein kinase B; blood coagulation; calcium-mediated signaling; cell migration; cellular response to insulin stimulus; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; focal adhesion formation; hyperosmotic response; innate immune response; insulin receptor signaling pathway; negative regulation of protein kinase activity; negative regulation of toll-like receptor signaling pathway; negative regulation of transforming growth factor beta receptor signaling pathway; nerve growth factor receptor signaling pathway; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; phosphoinositide-mediated signaling; platelet activation; positive regulation of release of sequestered calcium ion into cytosol; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of I-kappaB kinase/NF-kappaB cascade; regulation of mast cell degranulation; regulation of transcription, DNA-dependent; small GTPase mediated signal transduction; stimulatory C-type lectin receptor signaling pathway; synaptic transmission; T cell costimulation; T cell receptor signaling pathway; transcription, DNA-dependent; vascular endothelial growth factor receptor signaling pathway

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