Full Product Name
AFF4 Antibody
Product Synonym Names
AFF4; AF4/FMR2 family member 4; AF4/FMR2 family; member 4; AF5Q31; MCEF; Protein AF-5q31
Product Gene Name
anti-AFF4 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9UHB7
Species Reactivity
Human, Mouse
Specificity
AFF4 Antibody detects endogenous levels of total AFF4
Form/Format
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0mg/mL (lot specific)
Immunogen
A synthesized peptide derived from human AFF4
Preparation and Storage
Store at -20 degree C
Other Notes
Small volumes of anti-AFF4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-AFF4 antibody
Total protein Ab
Applications Tested/Suitable for anti-AFF4 antibody
Western Blot (WB)
Application Notes for anti-AFF4 antibody
Western Blot: 1:1000-3000
Western Blot (WB) of anti-AFF4 antibody
Western blot analysis AFF4 using NIH-3T3 whole cell lysates
NCBI/Uniprot data below describe general gene information for AFF4. It may not necessarily be applicable to this product.
NCBI Accession #
NP_055238.1
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NCBI GenBank Nucleotide #
NM_014423.3
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UniProt Primary Accession #
Q9UHB7
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UniProt Secondary Accession #
Q498B2; Q59FB3; Q6P592; Q8TDR1; Q9P0E4; B2RP19; B7WPD2[Other Products]
UniProt Related Accession #
Q9UHB7[Other Products]
Molecular Weight
38,823 Da
NCBI Official Full Name
AF4/FMR2 family member 4
NCBI Official Synonym Full Names
AF4/FMR2 family member 4
NCBI Official Symbol
AFF4??[Similar Products]
NCBI Official Synonym Symbols
MCEF; CHOPS; AF5Q31
??[Similar Products]
NCBI Protein Information
AF4/FMR2 family member 4
UniProt Protein Name
AF4/FMR2 family member 4
UniProt Synonym Protein Names
ALL1-fused gene from chromosome 5q31 protein; Protein AF-5q31; Major CDK9 elongation factor-associated protein
Protein Family
AF4/FMR2 family
UniProt Gene Name
AFF4??[Similar Products]
UniProt Synonym Gene Names
AF5Q31; MCEF; Protein AF-5q31??[Similar Products]
UniProt Entry Name
AFF4_HUMAN
NCBI Summary for AFF4
The protein encoded by this gene belongs to the AF4 family of transcription factors involved in leukemia. It is a component of the positive transcription elongation factor b (P-TEFb) complex. A chromosomal translocation involving this gene and MLL gene on chromosome 11 is found in infant acute lymphoblastic leukemia with ins(5;11)(q31;q31q23). [provided by RefSeq, Oct 2011]
UniProt Comments for AFF4
MCEF: a putative transcription factor. Component of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor b (P-TEFb). Genetic fusion of its gene with MLL has been observed in a subset of acute lymphoblastic leukemia patients. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Oncoprotein; Transcription factor
Chromosomal Location of Human Ortholog: 5q31
Cellular Component: transcription elongation factor complex
Molecular Function: protein binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter
Disease: Chops Syndrome
Research Articles on AFF4
1. AFF4 is positioned to make unexpected direct contacts with HIV Tat, and Tat enhances P-TEFb/CCNT1 affinity for AFF4.
Precautions
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Disclaimer
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