Full Product Name
AFF4 Antibody
Product Synonym Names
AF4/FMR2 family member 4
Product Gene Name
anti-AFF4 antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9UHB7
Purity/Purification
>95%, Protein G purified
Immunogen
Recombinant human AF4/FMR2 family member 4 protein (77-334AA)
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, PH 7.4
Conjugation
Non-conjugated
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-AFF4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-AFF4 antibody
Key component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by Key component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. In the SEC complex, AFF4 acts as a central scaffold that recruits other factors through direct interactions with ELL proteins (ELL, ELL2 or ELL3) and the P-TEFb complex. In case of infection by HIV-1 virus, the SEC complex is recruited by the viral Tat protein to stimulate viral gene expression.
Applications Tested/Suitable for anti-AFF4 antibody
ELISA (EIA)
Western Blot (WB) of anti-AFF4 antibody
Western Blot Positive WB detected in:Mouse liver tissue All lanes: AFF4 antibody at 2.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution predicted band size: 127,98,39 kDa observed band size: 39 kDa
NCBI/Uniprot data below describe general gene information for AFF4. It may not necessarily be applicable to this product.
NCBI Accession #
NP_055238.1
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NCBI GenBank Nucleotide #
NM_014423.3
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UniProt Primary Accession #
Q9UHB7
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UniProt Secondary Accession #
Q498B2; Q59FB3; Q6P592; Q8TDR1; Q9P0E4; B2RP19; B7WPD2[Other Products]
UniProt Related Accession #
Q9UHB7[Other Products]
Molecular Weight
38,823 Da
NCBI Official Full Name
AF4/FMR2 family member 4
NCBI Official Synonym Full Names
AF4/FMR2 family member 4
NCBI Official Symbol
AFF4??[Similar Products]
NCBI Official Synonym Symbols
MCEF; CHOPS; AF5Q31
??[Similar Products]
NCBI Protein Information
AF4/FMR2 family member 4
UniProt Protein Name
AF4/FMR2 family member 4
UniProt Synonym Protein Names
ALL1-fused gene from chromosome 5q31 protein; Protein AF-5q31; Major CDK9 elongation factor-associated protein
Protein Family
AF4/FMR2 family
UniProt Gene Name
AFF4??[Similar Products]
UniProt Synonym Gene Names
AF5Q31; MCEF; Protein AF-5q31??[Similar Products]
UniProt Entry Name
AFF4_HUMAN
NCBI Summary for AFF4
The protein encoded by this gene belongs to the AF4 family of transcription factors involved in leukemia. It is a component of the positive transcription elongation factor b (P-TEFb) complex. A chromosomal translocation involving this gene and MLL gene on chromosome 11 is found in infant acute lymphoblastic leukemia with ins(5;11)(q31;q31q23). [provided by RefSeq, Oct 2011]
UniProt Comments for AFF4
MCEF: a putative transcription factor. Component of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor b (P-TEFb). Genetic fusion of its gene with MLL has been observed in a subset of acute lymphoblastic leukemia patients. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Oncoprotein; Transcription factor
Chromosomal Location of Human Ortholog: 5q31
Cellular Component: transcription elongation factor complex
Molecular Function: protein binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter
Disease: Chops Syndrome
Research Articles on AFF4
1. AFF4 is positioned to make unexpected direct contacts with HIV Tat, and Tat enhances P-TEFb/CCNT1 affinity for AFF4.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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