Product Name
CCBE1, siRNA
Full Product Name
CCBE1 siRNA (Mouse)
Product Synonym Names
KIAA1983; Collagen and calcium-binding EGF domain-containing protein 1; Full of fluid protein homolog
Product Gene Name
CCBE1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q3MI99
Specificity
CCBE1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CCBE1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CCBE1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CCBE1 sirna
siRNA to inhibit CCBE1 expression using RNA interference
Applications Tested/Suitable for CCBE1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CCBE1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_848908.1
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NCBI GenBank Nucleotide #
NM_178793.4
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UniProt Primary Accession #
Q3MI99
[Other Products]
UniProt Secondary Accession #
Q5DTT5; Q8BFW1; Q8BMT1; A7MCU5[Other Products]
UniProt Related Accession #
Q3MI99[Other Products]
Molecular Weight
44,357 Da
NCBI Official Full Name
collagen and calcium-binding EGF domain-containing protein 1
NCBI Official Synonym Full Names
collagen and calcium binding EGF domains 1
NCBI Official Symbol
Ccbe1??[Similar Products]
NCBI Official Synonym Symbols
mKIAA1983; 4933426F18Rik; 9430093N24Rik
??[Similar Products]
NCBI Protein Information
collagen and calcium-binding EGF domain-containing protein 1
UniProt Protein Name
Collagen and calcium-binding EGF domain-containing protein 1
UniProt Synonym Protein Names
Full of fluid protein homolog
Protein Family
Collagen and calcium-binding EGF domain-containing protein
UniProt Gene Name
Ccbe1??[Similar Products]
UniProt Synonym Gene Names
Kiaa1983??[Similar Products]
UniProt Entry Name
CCBE1_MOUSE
UniProt Comments for CCBE1
CCBE1: Required for lymphangioblast budding and angiogenic sprouting from venous endothelium during embryogenesis. Defects in CCBE1 are the cause of Hennekam lymphangiectasia-lymphedema syndrome (HLLS). HLLS is a generalized lymph-vessels dysplasia characterized by intestinal lymphangiectasia with severe lymphedema of the limbs, genitalia and face. In addition, affected individuals have unusual facies and severe mental retardation. Belongs to the CCBE1 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Cellular Component: proteinaceous extracellular matrix; extracellular space; collagen; extracellular region
Molecular Function: collagen binding; protease binding; calcium ion binding
Biological Process: positive regulation of angiogenesis; multicellular organismal development; lymphangiogenesis; angiogenesis
Research Articles on CCBE1
1. Collagen domains of CCBE1 are crucial for the activation of VEGFC in vitro and in vivo. The EGF domains of CCBE1 are dispensable for regulation of VEGFC processing in vitro, however, they are necessary for full lymphangiogenic activity of CCBE1 in vivo.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
