Product Name
ITGA2B, siRNA
Full Product Name
ITGA2B siRNA (Human)
Product Synonym Names
GP2B; ITGAB; Integrin alpha-IIb; GPalpha IIb; GPIIb; Platelet membrane glycoprotein IIb; CD antigen CD41
Product Gene Name
ITGA2B sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P08514
Specificity
ITGA2B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ITGA2B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ITGA2B sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ITGA2B sirna
siRNA to inhibit ITGA2B expression using RNA interference
Applications Tested/Suitable for ITGA2B sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ITGA2B. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000410.2
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NCBI GenBank Nucleotide #
NM_000419.3
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UniProt Primary Accession #
P08514
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UniProt Secondary Accession #
O95366; Q14443; Q17R67; B2RCY8[Other Products]
UniProt Related Accession #
P08514[Other Products]
Molecular Weight
103,218 Da
NCBI Official Full Name
integrin alpha-IIb preproprotein
NCBI Official Synonym Full Names
integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41)
NCBI Official Symbol
ITGA2B??[Similar Products]
NCBI Official Synonym Symbols
GT; GTA; CD41; GP2B; HPA3; CD41B; GPIIb; BDPLT2; BDPLT16; PPP1R93
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NCBI Protein Information
integrin alpha-IIb
UniProt Protein Name
Integrin alpha-IIb
UniProt Synonym Protein Names
GPalpha IIb; GPIIb
UniProt Gene Name
ITGA2B??[Similar Products]
UniProt Synonym Gene Names
GP2B; ITGAB; GPIIb??[Similar Products]
UniProt Entry Name
ITA2B_HUMAN
NCBI Summary for ITGA2B
ITGA2B encodes integrin alpha chain 2b. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 2b undergoes post-translational cleavage to yield disulfide-linked light and heavy chains that join with beta 3 to form a fibronectin receptor expressed in platelets that plays a crucial role in coagulation. Mutations that interfere with this role result in thrombasthenia. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq, Jul 2008]
UniProt Comments for ITGA2B
ITGA2B: Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface. Belongs to the integrin alpha chain family. Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-IIb associates with beta-3. Directly interacts with RNF181. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral
Chromosomal Location of Human Ortholog: 17q21.32
Cellular Component: platelet alpha granule membrane; focal adhesion; cell surface; integral to plasma membrane; plasma membrane; integrin complex; external side of plasma membrane
Molecular Function: identical protein binding; protein binding; extracellular matrix binding; metal ion binding
Biological Process: integrin-mediated signaling pathway; platelet activation; axon guidance; extracellular matrix organization and biogenesis; platelet degranulation; cell-matrix adhesion; positive regulation of leukocyte migration; cell adhesion; blood coagulation
Disease: Glanzmann Thrombasthenia; Bleeding Disorder, Platelet-type, 16
Research Articles on ITGA2B
1. post-treatment of peptides derived from the full-length tail or from the turn motif did not reverse aIIb and aV integrin activation.
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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