Full Product Name
HAMSTER ANTI MOUSE CD339
Product Gene Name
anti-CD339 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (lot specific)
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation
Immunogen
Jagged1-expressing CHO cells.
Histology Positive Control Tissue
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Other Notes
Small volumes of anti-CD339 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD339 antibody
Hamster anti Mouse CD339 antibody, clone HMJ1-29 specifically recognizes CD339, otherwise known as Jagged1, one of the five major ligands of the Notch signaling pathway, which is activated through the binding of specific ligands to the Notch receptors Notch 1-4. The Notch signaling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell hematopoiesis, thymic T-cell development, and both tumor progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta-like protein 1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and the gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signaling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. Jagged1 signaling is implicated in cell-fate decisions during hematopoiesis, as well as in both early and late stages of mammalian cardiovascular development, and is involved in the inhibition of myoblast differentiation. Studies have shown a significant increase in the expression of Jagged1 in metastatic prostate cancer, compared with localized prostate cancer or benign prostatic tissues, implicating Jagged1 as a biomarker to facilitate their differentiation. In humans, mutations in the JAG1 gene are responsible for the autosomal dominant
Applications Tested/Suitable for anti-CD339 antibody
Immunohistology Frozen, Flow cytometry (FC/FACS)
Application Notes for anti-CD339 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Testing Data of anti-CD339 antibody
Mouse CD339 transfected cells stained with Hamster anti Mouse CD339 (MBS219863) following cell permeabilisation with Leucoperm?
NCBI/Uniprot data below describe general gene information for CD339. It may not necessarily be applicable to this product.
NCBI Accession #
NP_062020.1
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NCBI GenBank Nucleotide #
NM_019147.1
[Other Products]
UniProt Secondary Accession #
P70640[Other Products]
UniProt Related Accession #
Q63722[Other Products]
Molecular Weight
134,326 Da
NCBI Official Full Name
protein jagged-1
NCBI Official Synonym Full Names
jagged 1
NCBI Official Symbol
Jag1??[Similar Products]
NCBI Protein Information
protein jagged-1
UniProt Protein Name
Protein jagged-1
UniProt Synonym Protein Names
CD_antigen: CD339
UniProt Gene Name
Jag1??[Similar Products]
UniProt Synonym Gene Names
Jagged1??[Similar Products]
UniProt Entry Name
JAG1_RAT
NCBI Summary for CD339
ligand responsible for activating Notch1 [RGD, Feb 2006]
UniProt Comments for CD339
Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Enhances fibroblast growth factor-induced angiogenesis (in vitro). Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation. May regulate fibroblast growth factor-induced angiogenesis.
Research Articles on CD339
1. Although Jag1 shares a high degree of homology with Jag2 in the ectodomain region, BACE1 fails to cleave Jag2 effectively, indicating a selective cleavage of Jag1.
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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