Full Product Name
B-Raf (Phospho-Ser729) Antibody
Product Synonym Names
FLJ95109; 94 kDa B raf protein; B raf 1; B Raf proto oncogene serine threonine protein kinase; B Raf proto oncogene, serine/threonine kinase; B RAF1; B-Raf proto-oncogene serine/threonine-protein kinase (p94); BRAF 1; BRAF; BRAF_HUMAN; BRAF1; cRmil; MGC126806; MGC138284; Murine sarcoma viral (v-raf) oncogene homolog B1; Murine sarcoma viral v raf oncogene homolog B1; NS7; Oncogen BRAF; oncogene BRAF1; p94; Proto-oncogene B-Raf; Proto-oncogene c-Rmil; RAFB 1; RAFB1; RMIL; Serine/threonine-protein kinase B-raf; v raf murine sarcoma viral oncogene homolog B; v raf murine sarcoma viral oncogene homolog B1; v-Raf murine sarcoma viral oncogene homolog B1;
Product Gene Name
anti-B-Raf antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P15056
Species Reactivity
Human, Mouse, Rat
Specificity
Phospho-B-Raf (Phospho-Ser729) Antibody detects endogenous levels of B-Raf only when phosphorylated at Ser729
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Form/Format
In phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0mg/mL (lot specific)
Immunogen
A synthesized peptide derived from human B-Raf around the phosphorylation site of Ser729
Preparation and Storage
Store at -20 degree C.
Other Notes
Small volumes of anti-B-Raf antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-B-Raf antibody
Phospho-specific Ab
Applications Tested/Suitable for anti-B-Raf antibody
Western Blot (WB)
Application Notes for anti-B-Raf antibody
WB: 1:500-1:2000
Western Blot (WB) of anti-B-Raf antibody
Western blot analysis of Phospho-B-Raf (Ser729) in lysates of PC-12?, using Phospho-B-Raf (Ser729) Antibody(13089).
NCBI/Uniprot data below describe general gene information for B-Raf. It may not necessarily be applicable to this product.
NCBI Accession #
NP_004324.2
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NCBI GenBank Nucleotide #
NM_004333.5
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UniProt Primary Accession #
P15056
[Other Products]
UniProt Secondary Accession #
Q13878; Q3MIN6; Q9UDP8; Q9Y6T3; A4D1T4; B6HY61; B6HY62; B6HY63; B6HY64; B6HY65; B6HY66[Other Products]
UniProt Related Accession #
P15056[Other Products]
NCBI Official Full Name
serine/threonine-protein kinase B-raf isoform 1
NCBI Official Synonym Full Names
B-Raf proto-oncogene, serine/threonine kinase
NCBI Official Symbol
BRAF??[Similar Products]
NCBI Official Synonym Symbols
NS7; B-raf; BRAF1; RAFB1; B-RAF1
??[Similar Products]
NCBI Protein Information
serine/threonine-protein kinase B-raf
UniProt Protein Name
Serine/threonine-protein kinase B-raf
UniProt Synonym Protein Names
Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1
UniProt Gene Name
BRAF??[Similar Products]
UniProt Synonym Gene Names
BRAF1; RAFB1??[Similar Products]
NCBI Summary for B-Raf
This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017]
UniProt Comments for B-Raf
Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.
Research Articles on B-Raf
1. >>>BRAF V600E = TERT mutation alone >RAS mutation alone = wild-type genes.">This multicenter data analysis establishes a six-genotype genetic prognostic model for poor outcomes of papillary thyroid cancer with a risk order of genetic duet of BRAF V600E/RAS mutation and TERT mutation >>>>BRAF V600E = TERT mutation alone >RAS mutation alone = wild-type genes.
Precautions
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Disclaimer
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