Mouse Phenylalanine-4-hydroxylase (PAH) ELISA kit; PH; PKU; PKU1; ; phenylalanine hydroxylase

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of mouse PAH. No significant cross-reactivity or interference between mouse PAH and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of PAH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS946253 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the phenylalanine hydroxylase (PAH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PAH. The ELISA analytical biochemical technique of the MBS946253 kit is based on PAH antibody-PAH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PAH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PAH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PAH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PAH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PAH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAH bound in the initial step. The color development is stopped and the intensity of the color is measured.

51,900 Da

phenylalanine hydroxylase

phenylalanine-4-hydroxylase; phe-4-monooxygenase

Phenylalanine-4-hydroxylase

PAH??[Similar Products]

PH4H_MOUSE

PAH: phenylalanine hydroxylase that is the rate-limiting step in phenylalanine catabolism. Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria.

Protein type: Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Oxidoreductase; EC 1.14.16.1

Molecular Function: amino acid binding; protein homodimerization activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced pteridine as one donor, and incorporation of one atom of oxygen; iron ion binding; metal ion binding; phenylalanine 4-monooxygenase activity; oxidoreductase activity; cofactor binding; monooxygenase activity; catalytic activity

Biological Process: L-phenylalanine metabolic process; tyrosine biosynthetic process; L-phenylalanine catabolic process; tetrahydrobiopterin metabolic process; aromatic amino acid family metabolic process; metabolic process; pteridine and derivative metabolic process; protein amino acid hydroxylation

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