For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of CaN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS737072 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Calcineurin (CaN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CaN. The ELISA analytical biochemical technique of the MBS737072 kit is based on CaN antibody-CaN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CaN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CaN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: CaN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CaN antibody and an CaN-HRP conjugate. The assay sample and buffer are incubated together with CaN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CaN concentration since CaN from samples and CaN-HRP conjugate compete for the anti-CaN antibody . Since the number of sites is limited, as more sites are occupied by CaN from the sample, fewer sites are left to bind CaN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CaN concentration in each sample is interpolated from this standard curve.

Immunology

8,802 Da

FK506 binding protein 1B, 12.6 kDa

peptidyl-prolyl cis-trans isomerase FKBP1B; FKBP-1B; rotamase; FKBP-12.6; h-FKBP-12; calstabin 2; 12.6 kDa FKBP; PPIase FKBP1B; immunophilin FKBP12.6; FK506-binding protein 1B; FK506-binding protein 12.6; 12.6 kDa FK506-binding protein

Peptidyl-prolyl cis-trans isomerase FKBP1B

FKBP12.6; FKBP1L; FKBP9; OTK4; PPIase FKBP1B; 12.6 kDa FKBP; FKBP-12.6; FKBP-1B??[Similar Products]

FKB1B_HUMAN

The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. It is highly similar to the FK506-binding protein 1A. Its physiological role is thought to be in excitation-contraction coupling in cardiac muscle. There are two alternatively spliced transcript variants of this gene encoding different isoforms. [provided by RefSeq, Jul 2008]

FKBP1B: Has the potential to contribute to the immunosuppressive and toxic effects of FK506 and rapamycin. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Belongs to the FKBP-type PPIase family. FKBP1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Isomerase; EC 5.2.1.8

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: sarcoplasmic reticulum membrane; membrane; cytoplasm; cytosol; Z disc

Molecular Function: protein binding; FK506 binding; cyclic nucleotide binding; peptidyl-prolyl cis-trans isomerase activity; calcium channel inhibitor activity; ryanodine-sensitive calcium-release channel activity; receptor binding

Biological Process: positive regulation of sequestering of calcium ion; protein maturation via protein folding; positive regulation of axon regeneration; protein peptidyl-prolyl isomerization; cytosolic calcium ion homeostasis; negative regulation of heart rate; response to redox state; release of sequestered calcium ion by sarcoplasmic reticulum into cytosol; T cell proliferation; negative regulation of protein phosphatase type 2B activity; response to vitamin E; smooth muscle contraction; 'de novo' protein folding; response to hydrogen peroxide; insulin secretion; negative regulation of release of sequestered calcium ion into cytosol; action potential propagation; response to glucose stimulus; protein refolding; transmembrane transport

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