mcp-4; ccl13; mcp4; ccl-13

For Research Use Only. Not for use in diagnostic procedures.

E Coli

>98%, as determined by SDS-PAGE and HPLC

Recombinant MCP-4 was lyophilized from a 0.2 um filtered 20mM PB solution pH 7.5, 100mM NaCl.

The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

Small volumes of MCP-4 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Monocyte Chemoattractant Proteins 4 (MCP-4/CCL13) is member of a distinct, structurally-related subclass of CC chemokines. MCP-4/CCL13 is a major chemoattractants for eosinophils, basophils monocytes and T lymphocytes. The MCP protein family bind to specific G-protein- coupled receptors, initiating a signal cascade within the cell. Expression of MCP-4/CCL13 mRNA and protein is greater in the sputum, epithelium, submucosal inflammatory cells and bronchoalveolar lavage fluid of asthmatics than in healthy individuals. Involvement of MCPs has also been demonstrated in renal inflammation and atopic dermatitis and the expression of this protein is also correlated with enhanced inflammatory immune responses during immunotherapy

Cytokines

10,986 Da

C-C motif chemokine 13

MCP4; NCC1; SCYA13; MCP-4??[Similar Products]

CCL13_HUMAN

This antimicrobial gene is one of several Cys-Cys (CC) cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. This chemokine plays a role in accumulation of leukocytes during inflammation. It may also be involved in the recruitment of monocytes into the arterial wall during artherosclerosis. [provided by RefSeq, Sep 2014]

CCL13: Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens. By IL1/interleukin-1 and TNF. Widely expressed. Found in small intestine, thymus, colon, lung, trachea, stomach and lymph node. Low levels seen in the pulmonary artery smooth muscle cells. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted, signal peptide; Chemokine; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: extracellular space

Molecular Function: chemokine activity; receptor binding

Biological Process: cellular calcium ion homeostasis; regulation of cell shape; cell-cell signaling; eosinophil chemotaxis; immune response; cytoskeleton organization and biogenesis; inflammatory response; chemotaxis; signal transduction

? raised MCP-4 levels in symptomatic carotid atherosclerosis: an inflammatory link between platelet and monocyte activation .Cardiovasc Res, Mar 2010; 10.1093/cvr/cvq044.
? monocyte chemoattractant protein-4 (MCP-4) /ccl13 is highly expressed in cartilage from patients with rheumatoid arthritis .Rheumatology, Apr 2006; 45: 421 - 424.
? il-4 differentially regulates eotaxin and MCP-4 in lung epithelium and circulating mononuclear cells .Am J Physiol Lung Cell Mol Physiol, Nov 2001; 281: 1288.
? monocyte chemoattractant protein (mcp) -4 expression in the airways of patients with asthma. induction in epithelial cells and mononuclear cells by proinflammatory cytokines .Am. J. Respir. Crit. Care Med., Aug 2000; 162: 723 - 732.
? eosinophil chemotactic chemokines (eotaxin, eotaxin-2, rantes, monocyte chemoattractant protein-3 (mcp-3), and MCP-4), and c-c chemokine receptor 3 expression in bronchial biopsies from atopic and nonatopic (intrinsic) asthmatics .J. Immunol., Dec 1999; 163: 6321 - 6329.