Product Name
SLC22A11, siRNA
Full Product Name
SLC22A11 siRNA (Human)
Product Synonym Names
OAT4; Solute carrier family 22 member 11; Organic anion transporter 4
Product Gene Name
SLC22A11 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9NSA0
Specificity
SLC22A11 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SLC22A11 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of SLC22A11 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
SLC22A11 sirna
siRNA to inhibit SLC22A11 expression using RNA interference
Applications Tested/Suitable for SLC22A11 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for SLC22A11. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001294914.1
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NCBI GenBank Nucleotide #
NM_001307985.1
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UniProt Primary Accession #
Q9NSA0
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UniProt Secondary Accession #
Q53GR2; Q6ZP72; Q8NBU4; A8K426[Other Products]
UniProt Related Accession #
Q9NSA0[Other Products]
Molecular Weight
48,301 Da
NCBI Official Full Name
solute carrier family 22 member 11 isoform 2
NCBI Official Synonym Full Names
solute carrier family 22 (organic anion/urate transporter), member 11
NCBI Official Symbol
SLC22A11??[Similar Products]
NCBI Official Synonym Symbols
OAT4; hOAT4
??[Similar Products]
NCBI Protein Information
solute carrier family 22 member 11
UniProt Protein Name
Solute carrier family 22 member 11
UniProt Synonym Protein Names
Organic anion transporter 4
Protein Family
Solute carrier family
UniProt Gene Name
SLC22A11??[Similar Products]
UniProt Synonym Gene Names
OAT4??[Similar Products]
UniProt Entry Name
S22AB_HUMAN
NCBI Summary for SLC22A11
The protein encoded by this gene is involved in the sodium-independent transport and excretion of organic anions, some of which are potentially toxic. The encoded protein is an integral membrane protein and is found mainly in the kidney and in the placenta, where it may act to prevent potentially harmful organic anions from reaching the fetus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]
UniProt Comments for SLC22A11
SLC22A11: Mediates saturable uptake of estrone sulfate, dehydroepiandrosterone sulfate and related compounds. Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, multi-pass; Transporter; Transporter, SLC family; Membrane protein, integral; Cell surface
Chromosomal Location of Human Ortholog: 11q13.1
Cellular Component: integral to plasma membrane; apical plasma membrane; plasma membrane; external side of plasma membrane
Molecular Function: protein binding; inorganic anion exchanger activity; organic anion transmembrane transporter activity; sodium-independent organic anion transmembrane transporter activity
Biological Process: urate metabolic process; transmembrane transport; organic anion transport
Research Articles on SLC22A11
1. A common variant of OAT4/SLC22A11 is associated with renal underexcretion type gout in Japanese men.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
