Alpha-actinin-2; Alpha-actinin skeletal muscle isoform 2; F-actin cross-linking protein; ACTN2; Alpha-actinin-3; Alpha-actinin skeletal muscle isoform 3; F-actin cross-linking protein; ACTN3

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Actinin alpha-2/3 Antibody detects endogenous levels of total Actinin alpha-2/3 protein.

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

1 mg/ml (lot specific)

Stable at -20 degree C for at least 1 year.

Small volumes of anti-ACTN2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)

WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:20000

Immunofluorescence analysis of HeLa cells, using Actinin alpha-2/3 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using Actinin alpha-2/3 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HepG2, HeLa, and HUVEC cells, using Actinin alpha-2/3 Antibody. The lane on the right is blocked with the synthesized peptide.

103 kDa

actinin alpha 2

alpha-actinin-2

Alpha-actinin-2

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013]

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

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