Product Name
ABHD12, siRNA
Full Product Name
ABHD12 siRNA (Rat)
Product Synonym Names
Monoacylglycerol lipase ABHD12; 2-arachidonoylglycerol hydrolase; Abhydrolase domain-containing protein 12
Product Gene Name
ABHD12 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q6AYT7
Specificity
ABHD12 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat ABHD12 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ABHD12 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ABHD12 sirna
siRNA to inhibit ABHD12 expression using RNA interference
Applications Tested/Suitable for ABHD12 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ABHD12. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001019485.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001024314.1
[Other Products]
UniProt Primary Accession #
Q6AYT7
[Other Products]
UniProt Related Accession #
Q6AYT7[Other Products]
Molecular Weight
45,296 Da
NCBI Official Full Name
monoacylglycerol lipase ABHD12
NCBI Official Synonym Full Names
abhydrolase domain containing 12
NCBI Official Symbol
Abhd12??[Similar Products]
NCBI Protein Information
monoacylglycerol lipase ABHD12
UniProt Protein Name
Monoacylglycerol lipase ABHD12
UniProt Synonym Protein Names
2-arachidonoylglycerol hydrolaseCurated; Abhydrolase domain-containing protein 12Curated
Protein Family
Monoacylglycerol lipase
UniProt Gene Name
Abhd12??[Similar Products]
UniProt Entry Name
ABD12_RAT
UniProt Comments for ABHD12
ABHD12: Has 2-arachidonoylglycerol hydrolase activity. May be a regulator of endocannabinoid signaling pathways. Defects in ABHD12 are the cause of polyneuropathy hearing loss ataxia retinitis pigmentosa and cataract (PHARC). PHARC is a slowly progressive neurologic disorder with a variable phenotype resembling Refsum disease. Clinical features include sensorineural hearing loss, visual problems related to cataracts, retinitis pigmentosa, pes cavus, ataxic and/or spastic gait disturbances with a progressive sensorimotor peripheral neuropathy. Other features include hyporeflexia, hyperreflexia, extensor plantar responses. Belongs to the serine esterase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Hydrolase; EC 3.1.1.23
Cellular Component: integral to membrane
Molecular Function: acylglycerol lipase activity
Biological Process: phosphatidylserine catabolic process; ***** walking behavior; acylglycerol catabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
