AAT6; ACTSA; MYMY5

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG

Rabbit

Affinity Purification

Store at -20 degree C. Avoid freeze/thaw cycles.

Small volumes of anti-ACTA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Primary antibody

Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IP: 1:50 - 1:100

Western blot analysis of extracts of various cell lines, using ACTA2 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded mouse skin using ACTA2 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human tonsil using ACTA2 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver using ACTA2 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung using ACTA2 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse intestin using ACTA2 antibody at dilution of 1:100 (40x lens).

42kDa

actin, alpha 2, smooth muscle, aorta

actin, aortic smooth muscle

Actin, aortic smooth muscle

ACTSA; ACTVS??[Similar Products]

The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]

ACTA2: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Up-regulated in response to enterovirus 71 (EV71) infection. Belongs to the actin family.

Protein type: Contractile; Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10q23.31

Cellular Component: cytoplasm; cytosol; extracellular space; filopodium; lamellipodium; protein complex

Molecular Function: protein kinase binding

Biological Process: muscle contraction; response to virus

Disease: Aortic Aneurysm, Familial Thoracic 6; Moyamoya Disease 5; Multisystemic Smooth Muscle Dysfunction Syndrome

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