CHD8; ATP-dependent helicase CHD8; Axis duplication inhibitor; CHD-8; HELSNF1; KIAA1564; Duplin; Helicase with SNF2 domain 1

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

CHD8 Antibody detects endogenous levels of total CHD8

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

1.0mg/mL (lot specific)

Store at -20 degree C

Small volumes of anti-CHD8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Total protein Ab

Western Blot (WB)

Western Blot: 1:1000-3000

Western blot analysis CHD8 using HepG2 whole cell lysates

262,348 Da

chromodomain helicase DNA binding protein 8

chromodomain-helicase-DNA-binding protein 8

Chromodomain-helicase-DNA-binding protein 8

CHD-8??[Similar Products]

CHD8_HUMAN

This gene encodes a member of the chromodomain-helicase-DNA binding protein family, which is characterized by a SNF2-like domain and two chromatin organization modifier domains. The encoded protein also contains brahma and kismet domains, which are common to the subfamily of chromodomain-helicase-DNA binding proteins to which this protein belongs. This gene has been shown to function in several processes that include transcriptional regulation, epigenetic remodeling, promotion of cell proliferation, and regulation of RNA synthesis. Allelic variants of this gene are associated with autism spectrum disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2016]

CHD-8: DNA helicase that acts as a chromatin remodeling factor and regulates transcription. Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. Suppresses p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity. Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity. Negatively regulates CTNNB1- targeted gene expression by being recruited specifically to the promoter regions of several CTNNB1 responsive genes. Involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF. Acts as a suppressor of STAT3 activity by suppressing the LIF-induced STAT3 transcriptional activity. Also acts as a transcription activator via its interaction with ZNF143 by participating in efficient U6 RNA polymerase III transcription. Interacts with p53/TP53, histone H1, CTNNB1, CTCF and PIAS3. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components C17orf49, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, KAT8/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with CHD7. Belongs to the SNF2/RAD54 helicase family. CHD8 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.4.12; Helicase; Transcription factor

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: nucleoplasm; nucleus; protein complex

Molecular Function: ATP binding; beta-catenin binding; chromatin binding; DNA binding; DNA helicase activity; DNA-dependent ATPase activity; histone binding; methylated histone residue binding; p53 binding; protein binding

Biological Process: ATP-dependent chromatin remodeling; brain development; gut development; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of Wnt receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase III promoter; positive regulation of transcription, DNA-dependent; Wnt receptor signaling pathway through beta-catenin

Disease: Autism, Susceptibility To, 18

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