Full Product Name
CHD8 siRNA (Human)
Product Synonym Names
HELSNF1; KIAA1564; Chromodomain-helicase-DNA-binding protein 8; CHD-8; ATP-dependent helicase CHD8; Helicase with SNF2 domain 1
Product Gene Name
CHD8 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9HCK8
Specificity
CHD8 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CHD8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CHD8 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CHD8 sirna
siRNA to inhibit CHD8 expression using RNA interference
Applications Tested/Suitable for CHD8 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CHD8. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001164100.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001170629.1
[Other Products]
UniProt Primary Accession #
Q9HCK8
[Other Products]
UniProt Secondary Accession #
Q4G0D8; Q68DQ0; Q6DKH9; Q6P440; Q6ZNL7; Q8N3Z9; Q8NCY4; Q8TBR9; Q96F26[Other Products]
UniProt Related Accession #
Q9HCK8[Other Products]
Molecular Weight
262,348 Da
NCBI Official Full Name
chromodomain-helicase-DNA-binding protein 8 isoform 1
NCBI Official Synonym Full Names
chromodomain helicase DNA binding protein 8
NCBI Official Symbol
CHD8??[Similar Products]
NCBI Official Synonym Symbols
AUTS18; HELSNF1
??[Similar Products]
NCBI Protein Information
chromodomain-helicase-DNA-binding protein 8
UniProt Protein Name
Chromodomain-helicase-DNA-binding protein 8
UniProt Synonym Protein Names
ATP-dependent helicase CHD8; Helicase with SNF2 domain 1
Protein Family
Chromodomain-helicase-DNA-binding protein
UniProt Gene Name
CHD8??[Similar Products]
UniProt Synonym Gene Names
HELSNF1; KIAA1564; CHD-8??[Similar Products]
UniProt Entry Name
CHD8_HUMAN
NCBI Summary for CHD8
This gene encodes a DNA helicase that functions as a transcription repressor by remodeling chromatin structure. It binds beta-catenin and negatively regulates Wnt signaling pathway, which plays a pivotal role in vertebrate early development and morphogenesis. Mice lacking this gene exhibit early embryonic death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010]
UniProt Comments for CHD8
CHD-8: DNA helicase that acts as a chromatin remodeling factor and regulates transcription. Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. Suppresses p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity. Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity. Negatively regulates CTNNB1- targeted gene expression by being recruited specifically to the promoter regions of several CTNNB1 responsive genes. Involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF. Acts as a suppressor of STAT3 activity by suppressing the LIF-induced STAT3 transcriptional activity. Also acts as a transcription activator via its interaction with ZNF143 by participating in efficient U6 RNA polymerase III transcription. Interacts with p53/TP53, histone H1, CTNNB1, CTCF and PIAS3. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components C17orf49, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, KAT8/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with CHD7. Belongs to the SNF2/RAD54 helicase family. CHD8 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription factor; EC 3.6.4.12; Helicase
Chromosomal Location of Human Ortholog: 14q11.2
Cellular Component: nucleoplasm; protein complex; nucleus
Molecular Function: DNA-dependent ATPase activity; protein binding; DNA helicase activity; DNA binding; histone binding; p53 binding; beta-catenin binding; chromatin binding; methylated histone residue binding; ATP binding
Biological Process: negative regulation of Wnt receptor signaling pathway; transcription, DNA-dependent; in utero embryonic development; positive regulation of transcription, DNA-dependent; Wnt receptor signaling pathway through beta-catenin; negative regulation of transcription from RNA polymerase II promoter; DNA duplex unwinding; gut development; ATP-dependent chromatin remodeling; positive regulation of transcription from RNA polymerase III promoter; positive regulation of transcription from RNA polymerase II promoter; brain development; negative regulation of transcription, DNA-dependent
Disease: Autism, Susceptibility To, 18
Research Articles on CHD8
1. In the first detailed analysis in cancer, a marked loss of CHD8 expression and increased BORIS/CTCF ratio indicate frequent disruption of CTCF and its effector genes in PCa.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
