Product Name
Palmitoyl Protein Thioesterase 1 (PPT1), Recombinant Protein
Full Product Name
Recombinant Palmitoyl Protein Thioesterase 1 (PPT1)
Product Gene Name
PPT1 recombinant protein
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Matching Pairs
Unconjugated
Antibody: Palmitoyl Protein Thioesterase 1 (MBS2026884)
Immunogen: Palmitoyl Protein Thioesterase 1 (MBS2030895)
Matching Pairs
APC-CY7 Conjugated Antibody: Palmitoyl Protein Thioesterase 1 (PPT1) (MBS2078419)
Immunogen: Palmitoyl Protein Thioesterase 1 (MBS2030895)
Matching Pairs
PE Conjugated Antibody: Palmitoyl Protein Thioesterase 1 (PPT1) (MBS2078420)
Immunogen: Palmitoyl Protein Thioesterase 1 (MBS2030895)
Matching Pairs
APC Conjugated Antibody: Palmitoyl Protein Thioesterase 1 (PPT1) (MBS2078421)
Immunogen: Palmitoyl Protein Thioesterase 1 (MBS2030895)
Matching Pairs
Cy3 Conjugated Antibody: Palmitoyl Protein Thioesterase 1 (PPT1) (MBS2078422)
Immunogen: Palmitoyl Protein Thioesterase 1 (MBS2030895)
Matching Pairs
FITC Conjugated Antibody: Palmitoyl Protein Thioesterase 1 (PPT1) (MBS2078423)
Immunogen: Palmitoyl Protein Thioesterase 1 (MBS2030895)
Matching Pairs
HRP Conjugated Antibody: Palmitoyl Protein Thioesterase 1 (PPT1) (MBS2078424)
Immunogen: Palmitoyl Protein Thioesterase 1 (MBS2030895)
Matching Pairs
Biotin Conjugated Antibody: Palmitoyl Protein Thioesterase 1 (PPT1) (MBS2096233)
Immunogen: Palmitoyl Protein Thioesterase 1 (MBS2030895)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHH H SSGLVPRGSH MASMTGGQQM GRGSEF- DPP SPPPLVIWHG MGDSCCNPMS MGVIKKMVEK EIPGIYVLSL EIGKNMMEDV ENSFFLNVNV QVNMVCQILE KDPKLQQGYN AIGFSQGGQF LRAVAQRCPT PPMMTLISVG GQHQGVFGLP RCPGESSHIC DFIRKSLNAG AYSKLVQERL VQAQYWHDPI KESVYRNYSI FLADINQERC VNESYKKNLM ALKKFVMVKF FNDSIVDPVD SEWFGFYRSG QAKETIPLQE STLYTEDRLG LKKMDKAGK< /div>
Host
Host: E Coli
Source: Prokaryotic expression
Form/Format
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Concentration
8.1 (lot specific)
Predicted Molecular Mass
32.3kDa
Accurate Molecular Mass (KD)
32kDa
Endotoxin
<1.0EU per 1ug (determined by the LAL method)
Expression System
Prokaryotic expression
Tag
two N-terminal Tags, His-tag and T7-tag
Organism Species
Mus musculus (Mouse)
Usage
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.
Other Notes
Small volumes of PPT1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for PPT1 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
SDS-Page of PPT1 recombinant protein
NCBI/Uniprot data below describe general gene information for PPT1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001285024.1
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NCBI GenBank Nucleotide #
NM_001298095.1
[Other Products]
UniProt Secondary Accession #
Q6NL60; Q8MT82[Other Products]
UniProt Related Accession #
Q9W3C7[Other Products]
Molecular Weight
35,780 Da
NCBI Official Full Name
Palmitoyl-protein thioesterase 1, isoform B
NCBI Official Synonym Full Names
Palmitoyl-protein thioesterase 1
NCBI Official Symbol
Ppt1??[Similar Products]
NCBI Official Synonym Symbols
CG12108; DmelCG12108; DmPpt1; ppt1; PPT1
??[Similar Products]
NCBI Protein Information
CG12108 gene product from transcript CG12108-RB
UniProt Protein Name
Palmitoyl-protein thioesterase 1
UniProt Synonym Protein Names
Palmitoyl-protein hydrolase 1
Protein Family
Palmitoyl-protein thioesterase
UniProt Gene Name
Ppt1??[Similar Products]
UniProt Synonym Gene Names
PPT-1??[Similar Products]
UniProt Entry Name
PPT1_DROME
UniProt Comments for PPT1
Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides.
Research Articles on PPT1
1. Ppt1 has a role in synaptic vesicle exo- and endocytosis at motor nerve terminals of the Drosophila neuromuscular junction.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.