Full Product Name
ACTA2 antibody
Product Synonym Names
Actin aortic smooth muscle; Actin; aortic smooth muscle; AAT6; ACTSA; MYMY5
Product Gene Name
anti-ACTA2 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P62736
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Concentration
1 mg/ml (lot specific)
Immunogen
Recombinant human ACTA2 (3-377aa) purified from E. coli
Clone Note
Anti-human ACTA2 mAb, clone AT132D3, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ACTA2 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of anti-ACTA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ACTA2 antibody
Alpha-actin-2 also known as actin, aortic smooth muscle or alpha smooth muscle actin (alpha-SMA, SMactin, alpha-SM-actin, ASMA). Actin proteins are major components of the eukaryotic cytoskeleton. At least six vertebrate actin isoforms have been identified. The cytoplasmic beta- and gamma-actin proteins are referred to as non-muscle actin proteins as they are predominantly expressed in non-muscle cells where they control cell structure and motility. Actin has major functions. Firstly, F-actin polymers form microfilaments - polar intracellular tracks for kinesin motor proteins, allowing the transport of vesicles, organelles and other cargo. Actin is a component of the cytoskeleton and links to alpha-actinin, E-cadherin and beta-catenin at adherens junctions. actin has a role in cell motility through polymerization and depolymerization of fibrils.
Product Categories/Family for anti-ACTA2 antibody
Cytoskeleton; Actin & Myosin Related
Applications Tested/Suitable for anti-ACTA2 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-ACTA2 antibody
WB: 1:1000.
Western Blot (WB) of anti-ACTA2 antibody
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTA2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: SW480 cell lysate Lane 3.: HeLa cell lysate
Immunofluorescence (IF) of anti-ACTA2 antibody
ICC/IF analysis of ACTA2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACTA2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
NCBI/Uniprot data below describe general gene information for ACTA2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001604
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NCBI GenBank Nucleotide #
NM_001613.3
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UniProt Primary Accession #
P62736
[Other Products]
UniProt Secondary Accession #
P03996; P04108; Q6FI19; B2R8A4[Other Products]
UniProt Related Accession #
P62736[Other Products]
Molecular Weight
42,009 Da
NCBI Official Full Name
actin, aortic smooth muscle
NCBI Official Synonym Full Names
actin, alpha 2, smooth muscle, aorta
NCBI Official Symbol
ACTA2??[Similar Products]
NCBI Official Synonym Symbols
ACTSA
??[Similar Products]
NCBI Protein Information
actin, aortic smooth muscle
UniProt Protein Name
Actin, aortic smooth muscle
UniProt Synonym Protein Names
Alpha-actin-2; Cell growth-inhibiting gene 46 protein
UniProt Gene Name
ACTA2??[Similar Products]
UniProt Synonym Gene Names
ACTSA; ACTVS??[Similar Products]
NCBI Summary for ACTA2
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a smooth muscle actin that is involved in vascular contractility and blood pressure homeostasis. Mutations in this gene cause a variety of vascular diseases, such as thoracic aortic disease, coronary artery disease, stroke, and Moyamoya disease, as well as multisystemic smooth muscle dysfunction syndrome. [provided by RefSeq, Sep 2017]
UniProt Comments for ACTA2
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Product References and Citations for anti-ACTA2 antibody
Herman IM. et al. (1993) Curr Opin Cell Biol. 5 (1): 48-55.; Guo DC., et al. (2009) Am J Hum Genet. 84 (5): 617-627.
Research Articles on ACTA2
1. our results seemed to justify the conclusion that ACTA2 did not play a significant role in the pathogenesis of BAV aortopathy.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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