Full Product Name
Anti-ACTA2 Rabbit Monoclonal Antibody
Product Synonym Names
alpha smooth muscle Actin Alpha-actin-2; Cell growth-inhibiting gene 46 protein
Product Gene Name
anti-ACTA2 antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P62736
Species Reactivity
Human, Mouse
Purity/Purification
Affinity purified using protein A
Form/Format
Colorless liquid
In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Immunogen
A synthetic peptide corresponding to the N-terminus of human alpha smooth muscle Actin
Preparation and Storage
Store at -20 degree C for 12 months.
The antibody solution should be gently mixed before use.
Other Notes
Small volumes of anti-ACTA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ACTA2 antibody
Rabbit monoclonal antibody to detect ACTA2 in human and mouse samples.
Actins are highly conserved proteins expressed in all eucaryotic cells. Actin filaments form part of the cytoskeleton and play essential roles in regulating cell shape and movement. Six distinct actin isotypes have been identified in mammalian cells. Each is encoded by a separated gene and is expressed in a developmentally regulated and tissue-specific manner; alpha and beta cytoplasmic actins are expressed in a wide variety of cells; whereas; expression of alpha skeletal; alpha cardiac; alpha vascular; and gamma enteric actins are more restricted to specialized muscle cell type. Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Furthermore; expression of smooth muscle alpha actin is regulated by hormones; cell proliferation; and altered by pathological conditions including oncogenic transformation and atherosclerosis.
Applications Tested/Suitable for anti-ACTA2 antibody
Immunohistochemistry (IHC); Western Blot (WB)
Application Notes for anti-ACTA2 antibody
IHC: 1:1000-1:2500 dilution
WB: 1:1000- 1:2000 dilution
Testing Data of anti-ACTA2 antibody
NCBI/Uniprot data below describe general gene information for ACTA2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001135417.1
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NCBI GenBank Nucleotide #
NM_001141945.2
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UniProt Primary Accession #
P62736
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UniProt Secondary Accession #
P03996; P04108; Q6FI19; B2R8A4[Other Products]
UniProt Related Accession #
P62736[Other Products]
Molecular Weight
42,009 Da
NCBI Official Full Name
actin, aortic smooth muscle
NCBI Official Synonym Full Names
actin, alpha 2, smooth muscle, aorta
NCBI Official Symbol
ACTA2??[Similar Products]
NCBI Official Synonym Symbols
AAT6; ACTSA; MYMY5
??[Similar Products]
NCBI Protein Information
actin, aortic smooth muscle
UniProt Protein Name
Actin, aortic smooth muscle
UniProt Synonym Protein Names
Alpha-actin-2; Cell growth-inhibiting gene 46 protein
UniProt Gene Name
ACTA2??[Similar Products]
UniProt Synonym Gene Names
ACTSA; ACTVS??[Similar Products]
NCBI Summary for ACTA2
The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]
UniProt Comments for ACTA2
ACTA2: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Up-regulated in response to enterovirus 71 (EV71) infection. Belongs to the actin family.
Protein type: Contractile; Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 10q23.31
Cellular Component: cytoplasm; cytosol; extracellular space; filopodium; lamellipodium; protein complex
Molecular Function: protein kinase binding
Biological Process: muscle contraction; response to virus
Disease: Aortic Aneurysm, Familial Thoracic 6; Moyamoya Disease 5; Multisystemic Smooth Muscle Dysfunction Syndrome
Research Articles on ACTA2
1. Two novel actin alpha 2 mutations (N117I and L348R) were identified in each familial non-syndromatic thoracic aortic aneurysm proband separately, and an additional novel actin alpha 2 mutation (Y168N) was identified in one patient with sporadic non-syndromatic thoracic aortic aneurysm.
Precautions
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