Full Product Name
Rabbit polyclonal to Human Akt3
Product Synonym Names
Akt3 Rabbit pab Antibody
Product Gene Name
anti-AKT3 antibody
[Similar Products]
Product Synonym Gene Name
AKT3; DKFZp434N0250; PKB-GAMMA; PKBG; PRKBG; RAC-PK-gamma; RAC-gamma; STK-2[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 1; NC_000001.10 (243651535..244006886, complement). Location: 1q44
3D Structure
ModBase 3D Structure for Q9Y243
Species Reactivity
Human, mouse, rat
Purity/Purification
Affinity purified
Immunogen
A Fusion protein of human Akt3
Storage Buffer
PBS with 0.1% Sodium Azide, 50% Glycerol
Preparation and Storage
Upon delivery aliquot and store at -20 degree C or -80 degree C
Other Notes
Small volumes of anti-AKT3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-AKT3 antibody
Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3alpha and beta (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3beta-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip (15) and p21 Waf1/CIP1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19).
Applications Tested/Suitable for anti-AKT3 antibody
Western Blot (WB)
Application Notes for anti-AKT3 antibody
WB: 1/500-1000
Western Blot (WB) of anti-AKT3 antibody
Western blot analysis of extracts of MCF-7 cells, using AKT3 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
NCBI/Uniprot data below describe general gene information for AKT3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_005456.1
[Other Products]
NCBI Related Accession #
Human, mouse, ratNP_859029.1[Other Products]
NCBI GenBank Nucleotide #
NM_005465.3
[Other Products]
UniProt Primary Accession #
Q9Y243
[Other Products]
UniProt Secondary Accession #
Q0VAA6; Q5VTI1; Q5VTI2; Q96QV3; Q9UFP5[Other Products]
UniProt Related Accession #
Q9Y243[Other Products]
Molecular Weight
55,775 Da
NCBI Official Full Name
RAC-gamma serine/threonine-protein kinase isoform 1
NCBI Official Synonym Full Names
v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
NCBI Official Symbol
AKT3??[Similar Products]
NCBI Official Synonym Symbols
PKBG; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma
??[Similar Products]
NCBI Protein Information
RAC-gamma serine/threonine-protein kinase; PKB gamma; RAC-gamma serine/threonine protein kinase
UniProt Protein Name
RAC-gamma serine/threonine-protein kinase
UniProt Synonym Protein Names
Protein kinase Akt-3; Protein kinase B gamma; PKB gamma; RAC-PK-gamma; STK-2
Protein Family
RAC-gamma serine/threonine-protein kinase
UniProt Gene Name
AKT3??[Similar Products]
UniProt Synonym Gene Names
PKBG; PKB gamma??[Similar Products]
UniProt Entry Name
AKT3_HUMAN
Product References and Citations for anti-AKT3 antibody
1. Franke, T.F. et al. (1997) Cell 88, 435-7.
2. Burgering, B.M. and Coffer, P.J. (1995) Nature 376, 599-602.
3. Franke, T.F. et al. (1995) Cell 81, 727-36.
4. Alessi, D.R. et al. (1996) EMBO J 15, 6541-51.
5. Sarbassov, D.D. et al. (2005) Science 307, 1098-101.
Precautions
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Disclaimer
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