Product Name
Nephrocystin-1 (Nphp1), Recombinant Protein
Full Product Name
Recombinant Mouse Nephrocystin-1 (Nphp1)
Product Gene Name
Nphp1 recombinant protein
[Similar Products]
Product Synonym Gene Name
Nphp1[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
1-687aa, full length protein
Sequence
MLARRPRDPL QALRRRGQEL KLQVDSLVTE SQLTGALEPS KRREIYQRCI QLKQAVDENK NTLQKLNKAD EAAPVGNYEQ RKEEEHSLLE KLACQLQELA VSISRKDALK VEAHSDKEED DTTEDDEEET GGEEEESEGD GEGKQEQASP KQAETETVTY IALGDFAAQQ TGDLTFKKGD VLHIIEKKPD GWWLAKDAEG VEGLIPRTYL EPYNKEDKLE SSEGSEEGGE EDGEEDVEVV DETADGAQVK QRTDSHWSAV RKAISEQINT VDVLATMGAI PAGFRPSTLS QLLDEAGNQF RASYFLQPEL TTSQLAFRDL TWDAKAGTIM SRPSRVSLIL TLWSCKMIPL PGTSIQVLSR HIRLCLFDGS KVLSNIHTVR AVWQPKKPKT WTFSPQVTGI LPCLLDGDCF IRSNSSTPDL GILFELGISY IRNSTGERGE LSCGWVFLKL FDASGVPIPA KTYELFLNGG TPYEKGVEVD PSVSRRAQGS VFRQMISVRR QPQLLVKLRS LNRRSRAMLS LLPETLVGSM CSAHLLIFYR QILGDVLLRD RTNLQSADLI SHPVLATFPL LLEQPDVMDA LRSSWSEKES TLKRAEKRDK ELLKAEFLLV YHDCVLPLLH STLLPPFRWA EEETEAARWK AIADFLKQSR ENEGSLKALL SPDGVHKPFD LSEQTFDFLG EIRKNSG
3D Structure
ModBase 3D Structure for Q9QY53
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
>85% (SDS-PAGE) (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Storage Buffer
Tris-based buffer, 50% glycerol
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of Nphp1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
Nphp1 recombinant protein
This gene encodes a protein with src homology domain 3 (SH3) patterns. This protein interacts with Crk-associated substrate, and it appears to function in the control of cell division, as well as in cell-cell and cell-matrix adhesion signaling, likely as part of a multifunctional complex localized in actin- and microtubule-based structures. Mutations in this gene cause familial juvenile nephronophthisis type 1, a kidney disorder involving both tubules and glomeruli. Defects in this gene are also associated with Senior-Loken syndrome type 1, also referred to as juvenile nephronophthisis with Leber amaurosis, which is characterized by kidney and eye disease, and with Joubert syndrome type 4, which is characterized by cerebellar ataxia, oculomotor apraxia, psychomotor delay and neonatal breathing abnormalities, sometimes including retinal dystrophy and renal disease. Multiple transcript variants encoding different isoforms have been found for this gene.
NCBI/Uniprot data below describe general gene information for Nphp1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_058598.1
[Other Products]
NCBI GenBank Nucleotide #
NM_016902.4
[Other Products]
UniProt Primary Accession #
Q9QY53
[Other Products]
UniProt Secondary Accession #
Q9D7G8; Q9QZP0; Q9WUZ2[Other Products]
UniProt Related Accession #
Q9QY53[Other Products]
Molecular Weight
77,035 Da
NCBI Official Full Name
nephrocystin-1 isoform a
NCBI Official Synonym Full Names
nephronophthisis 1 (juvenile) homolog (human)
NCBI Official Symbol
Nphp1??[Similar Products]
NCBI Protein Information
nephrocystin-1
UniProt Protein Name
Nephrocystin-1
Protein Family
Nephrocystin
UniProt Gene Name
Nphp1??[Similar Products]
UniProt Synonym Gene Names
Nph1??[Similar Products]
UniProt Comments for Nphp1
Together with BCAR1 it may play a role in the control of epithelial cell polarity. Involved in the organization of apical junctions in kidney cells, together with NPHP4 and RPGRIP1L/NPHP8. Does not seem to be strictly required for ciliogenesis. Seems to help to recruit PTK2B/PYK2 to cell matrix adhesions, thereby initiating phosphorylation of PTK2B/PYK2 and PTK2B/PYK2-dependent signaling. May play a role in the regulation of intraflagellar transport (IFT) during cilia assembly. Required for normal retina development. In connecting photoreceptor cilia influences the movement of some IFT proteins such as IFT88 and WDR19. Involved in spermatogenesis; required for the differentiation of early elongating spermatids into spermatozoa.
Research Articles on Nphp1
1. PC-1 polyproline motif interacts with the SH3 domain of NPHP1.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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