Product Name
ACTA2 / Smooth Muscle Actin, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human ACTA2 / Smooth Muscle Actin
Product Synonym Names
Anti-ACTA2 / Smooth Muscle Actin Antibody (N-Terminus) IHC-plus; ACTVS; Alpha-actin-2; Alpha-cardiac actin; AAT6; Actin; aortic smooth muscle; ACTSA; Smooth Muscle Actin; MYMY5; Human ACTA2; Smooth Muscle Actin
Product Gene Name
anti-ACTA2 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P62736
Species Reactivity
Bat, Gibbon, Bovine, Chicken, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Orangutan, Pig, Rabbit, Rat
Specificity
Human ACTA2. Variants NP_001604.1 and NP_001135417.1 encode the same protein.
Purity/Purification
Immunoaffinity Purified
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Concentration
0.5 mg/ml (lot specific)
Immunogen Description
Synthetic peptide EEEDSTALVC from the N-terminus of human ACTA2 (NP_001604.1; NP_001135417.1).
Immunogen Type
Synthetic peptide
Immunogen
ACTA2 / Smooth Muscle Actin antibody was raised against synthetic peptide EEEDSTALVC from the N-terminus of human ACTA2 (NP_001604.1; NP_001135417.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Turkey, Chicken, Platypus (100%); Xenopus, Seq squirt, Phytophthora, Tunicate, Shewanella (80%).
Antigen Modification
N-Terminus
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Other Notes
Small volumes of anti-ACTA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ACTA2 antibody
Actin alpha 2, the human aortic smooth muscle actin gene, is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.
Applications Tested/Suitable for anti-ACTA2 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-ACTA2 antibody
ELISA (1:4000), IHC-P (2.5 ug/ml), WB (0.3 - 1 ug/ml)
Immunohistochemistry (IHC) of anti-ACTA2 antibody
Anti-ACTA2 antibody IHC of human colon, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

Immunohistochemistry (IHC) of anti-ACTA2 antibody
Anti-ACTA2 antibody IHC of human vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

Western Blot (WB) of anti-ACTA2 antibody
Antibody staining (0.1 ug/ml) of Human Duodenum lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

NCBI/Uniprot data below describe general gene information for ACTA2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001604.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001613.2
[Other Products]
UniProt Primary Accession #
P62736
[Other Products]
UniProt Secondary Accession #
P03996; P04108; Q6FI19; B2R8A4[Other Products]
UniProt Related Accession #
P62736[Other Products]
Molecular Weight
42,009 Da
NCBI Official Full Name
actin, aortic smooth muscle
NCBI Official Synonym Full Names
actin, alpha 2, smooth muscle, aorta
NCBI Official Symbol
ACTA2??[Similar Products]
NCBI Official Synonym Symbols
AAT6; ACTSA; MYMY5
??[Similar Products]
NCBI Protein Information
actin, aortic smooth muscle; alpha-cardiac actin; cell growth-inhibiting gene 46 protein
UniProt Protein Name
Actin, aortic smooth muscle
UniProt Synonym Protein Names
Alpha-actin-2; Cell growth-inhibiting gene 46 protein
UniProt Gene Name
ACTA2??[Similar Products]
UniProt Synonym Gene Names
ACTSA; ACTVS??[Similar Products]
UniProt Entry Name
ACTA_HUMAN
Precautions
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Disclaimer
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