Mouse Monoclonal [clone 1H8] (IgG1) to Human ACTA2 / Smooth Muscle Actin; Human ACTA2 / Smooth Muscle Actin; ACTVS; Alpha-actin-2; Alpha-cardiac actin; AAT6; Actin; aortic smooth muscle; ACTSA; Smooth Muscle Actin; MYMY5

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG1

1H8

Mouse

Human ACTA2 / Smooth Muscle Actin

Ascites

Ascites, 0.03% sodium azide

1 mg/ml (lot specific)

Long term: -20 degree C
Short term: 4 degree C
Avoid freeze-thaw cycles.

Small volumes of anti-ACTA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

ACTA2 Antibody, ACTVS Antibody, Alpha-actin-2 Antibody, Alpha-cardiac actin Antibody, AAT6 Antibody, Actin, aortic smooth muscle Antibody, ACTSA Antibody, Smooth Muscle Actin Antibody, MYMY5 Antibody Description: ACTA2 / Smooth Muscle Actin belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle.

Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)

ELISA (1:10000)
Flow Cytometry (1:200 - 1:400)
ICC (1:200 - 1:1000)
IF (1:1000)
IHC-P (2.5 - 5 ug/ml)
WB (1:500 - 1:2000)

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunofluorescence of NIH/3T3 cells using ACTA2 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Western blot using ACTA2 mouse monoclonal antibody against HeLa (1), Jurkat (2), HepG2 (3), MCF-7 (4), A431 (5), A549 (6), PC-12 (7), NIH/3T3 (8) and Cos7 (9) cell lysate.

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

42,009 Da

actin, alpha 2, smooth muscle, aorta

actin, aortic smooth muscle

Actin, aortic smooth muscle

ACTSA; ACTVS??[Similar Products]

ACTA_HUMAN

The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]

ACTA2: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Up-regulated in response to enterovirus 71 (EV71) infection. Belongs to the actin family.

Protein type: Cytoskeletal; Contractile; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10q23.3

Cellular Component: cytoplasm; cytoskeleton; cytosol; extracellular space; filopodium; lamellipodium; protein complex; smooth muscle contractile fiber

Molecular Function: ATP binding; protein kinase binding

Biological Process: muscle contraction; regulation of blood pressure; response to virus; vascular smooth muscle contraction

Disease: Aortic Aneurysm, Familial Thoracic 6; Moyamoya Disease 5; Multisystemic Smooth Muscle Dysfunction Syndrome

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