Product Name
ACTA2 / Smooth Muscle Actin, Monoclonal Antibody
Full Product Name
Mouse Monoclonal (IgG,k) to Human ACTA2 / Smooth Muscle Actin
Product Synonym Names
Anti-ACTA2 / Smooth Muscle Actin Antibody IHC-plus; ACTVS; Alpha-actin-2; Alpha-cardiac actin; AAT6; Actin; aortic smooth muscle; ACTSA; Smooth Muscle Actin; MYMY5; Human ACTA2; Smooth Muscle Actin
Product Gene Name
anti-ACTA2 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P62736
Specificity
Recognizes human Actin, Smooth Muscle. Staining pattern: Cytoplasmic.
Purity/Purification
Ascites
Form/Format
PBS, pH 7.4, 1% BSA, <0.1% sodium azide.
Immunogen Description
Human Actin, Smooth Muscle.
Immunogen
ACTA2 / Smooth Muscle Actin antibody was raised against human Actin, Smooth Muscle.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months
Other Notes
Small volumes of anti-ACTA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ACTA2 antibody
Actin alpha 2, the human aortic smooth muscle actin gene, is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.
Applications Tested/Suitable for anti-ACTA2 antibody
Immunohistochemistry (IHC - Paraffin; IHC - Frozen)
Application Notes for anti-ACTA2 antibody
IHC-Fr (1:10 - 1:50), IHC-P (1:50)
Usage: Suitable for use in Immunohistochemistry. Immunohistochemistry (Paraffin, frozen): 1:10-1:50.
Immunohistochemistry (IHC) of anti-ACTA2 antibody
Anti-Smooth Muscle Actin antibody IHC of human prostate, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
NCBI/Uniprot data below describe general gene information for ACTA2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001604.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001613.2
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UniProt Primary Accession #
P62736
[Other Products]
UniProt Secondary Accession #
P03996; P04108; Q6FI19; B2R8A4[Other Products]
UniProt Related Accession #
P62736[Other Products]
Molecular Weight
42,009 Da
NCBI Official Full Name
actin, aortic smooth muscle
NCBI Official Synonym Full Names
actin, alpha 2, smooth muscle, aorta
NCBI Official Symbol
ACTA2??[Similar Products]
NCBI Official Synonym Symbols
AAT6; ACTSA; MYMY5
??[Similar Products]
NCBI Protein Information
actin, aortic smooth muscle; alpha-cardiac actin; cell growth-inhibiting gene 46 protein
UniProt Protein Name
Actin, aortic smooth muscle
UniProt Synonym Protein Names
Alpha-actin-2; Cell growth-inhibiting gene 46 protein
UniProt Gene Name
ACTA2??[Similar Products]
UniProt Synonym Gene Names
ACTSA; ACTVS??[Similar Products]
UniProt Entry Name
ACTA_HUMAN
NCBI Summary for ACTA2
The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]
UniProt Comments for ACTA2
ACTA2: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Up-regulated in response to enterovirus 71 (EV71) infection. Belongs to the actin family.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal; Contractile
Chromosomal Location of Human Ortholog: 10q23.3
Cellular Component: extracellular space; smooth muscle contractile fiber; protein complex; cytoplasm; cytosol; actin cytoskeleton
Molecular Function: protein kinase binding; ATP binding
Biological Process: muscle contraction; regulation of blood pressure; response to virus; vascular smooth muscle contraction
Disease: Multisystemic Smooth Muscle Dysfunction Syndrome; Moyamoya Disease 5; Aortic Aneurysm, Familial Thoracic 6
Research Articles on ACTA2
1. Sphingosylphosphorylcholine stimulates alpha-SMA protein expression and human lung fibroblast mediated collagen gel contraction via S1P2 receptor.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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