Full Product Name
SGCG siRNA (Human)
Product Synonym Names
Gamma-sarcoglycan; Gamma-SG; 35 kDa dystrophin-associated glycoprotein; 35DAG
Product Gene Name
SGCG sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q13326
Specificity
SGCG siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SGCG gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of SGCG sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
SGCG sirna
siRNA to inhibit SGCG expression using RNA interference
Applications Tested/Suitable for SGCG sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for SGCG. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000222.1
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NCBI GenBank Nucleotide #
NM_000231.2
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UniProt Primary Accession #
Q13326
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UniProt Secondary Accession #
Q32M32; Q5T9J6[Other Products]
UniProt Related Accession #
Q13326[Other Products]
Molecular Weight
32,379 Da
NCBI Official Full Name
gamma-sarcoglycan
NCBI Official Synonym Full Names
sarcoglycan, gamma (35kDa dystrophin-associated glycoprotein)
NCBI Official Symbol
SGCG??[Similar Products]
NCBI Official Synonym Symbols
A4; MAM; DMDA; SCG3; TYPE; DAGA4; DMDA1; LGMD2C; SCARMD2
??[Similar Products]
NCBI Protein Information
gamma-sarcoglycan
UniProt Protein Name
Gamma-sarcoglycan
UniProt Synonym Protein Names
35 kDa dystrophin-associated glycoprotein; 35DAG
Protein Family
Gamma-sarcoglycan
UniProt Gene Name
SGCG??[Similar Products]
UniProt Synonym Gene Names
Gamma-SG; 35DAG??[Similar Products]
UniProt Entry Name
SGCG_HUMAN
NCBI Summary for SGCG
This gene encodes gamma-sarcoglycan, one of several sarcolemmal transmembrane glycoproteins that interact with dystrophin. The dystrophin-glycoprotein complex (DGC) spans the sarcolemma and is comprised of dystrophin, syntrophin, alpha- and beta-dystroglycans and sarcoglycans. The DGC provides a structural link between the subsarcolemmal cytoskeleton and the extracellular matrix of muscle cells. Defects in the encoded protein can lead to early onset autosomal recessive muscular dystrophy, in particular limb-girdle muscular dystrophy, type 2C (LGMD2C). [provided by RefSeq, Oct 2008]
UniProt Comments for SGCG
SGCG: Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix. Defects in SGCG are the cause of limb-girdle muscular dystrophy type 2C (LGMD2C). LGMD2C is characterized by progressive muscle wasting from early childhood. Belongs to the sarcoglycan beta/delta/gamma/zeta family.
Protein type: Dystrophin complex; Membrane protein, integral
Chromosomal Location of Human Ortholog: 13q12
Cellular Component: cytoskeleton; cytoplasm; plasma membrane; integral to membrane; sarcoglycan complex; sarcolemma
Molecular Function: protein binding
Biological Process: cardiac muscle development; muscle development; heart contraction; muscle cell development
Disease: Muscular Dystrophy, Limb-girdle, Type 2c
Research Articles on SGCG
1. A report of two siblings with severe childhood onset limb-girdle muscular dystrophy type 2C supports the theory that the mutation G787A in the SGCG gene is a founder mutation.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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